Coupling of antibodies with liposomes

Leaf Huang, Anthony Huang, Stephen Kennel

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Liposomes containing surface attached (covalently in most cases) antibody molecules have been termed "immunoliposomes". 1 As can be seen from other chapters in this book, there are basically two strategies for the covalent coupling of antibody with liposomes. In the first, native or modified antibody molecules are coupled to preformed liposomes containing suitable functional groups in the presence of coupling reagents. In the second, antibody molecules are first covalently modified with hydrophobic groups, the resulting amphipathic antibody molecules are then incorporated into liposomc membranes. The method used in our laboratory belongs to the latter type. We will describe the procedures for the covalent modification of antibody with fatty acids and then discuss the methods for the incorporation of fatty acyl antibodies into liposome bilayers. Encapsulation of solutes into immunoliposomcs will also be discussed since it is directly related to one of the major purposes of antibody coupling to liposomes, i.e., target-specific drug delivery. Finally, the interaction of immunoliposomes with cultured cells and some potential applications of these liposomes in medicine and biology will be discussed.

Original languageEnglish (US)
Title of host publicationLiposome Technology Volume III
Subtitle of host publicationTargeted Drug Delivery and Biological Interaction
PublisherCRC Press
Pages51-62
Number of pages12
ISBN (Electronic)9781351082563
ISBN (Print)0849353181, 9781315895017
DOIs
StatePublished - Jan 1 2018

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Liposomes
Antibodies
Cultured Cells
Fatty Acids
Medicine
Membranes
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Huang, L., Huang, A., & Kennel, S. (2018). Coupling of antibodies with liposomes. In Liposome Technology Volume III: Targeted Drug Delivery and Biological Interaction (pp. 51-62). CRC Press. https://doi.org/10.1201/9781351074117

Coupling of antibodies with liposomes. / Huang, Leaf; Huang, Anthony; Kennel, Stephen.

Liposome Technology Volume III: Targeted Drug Delivery and Biological Interaction. CRC Press, 2018. p. 51-62.

Research output: Chapter in Book/Report/Conference proceedingChapter

Huang, L, Huang, A & Kennel, S 2018, Coupling of antibodies with liposomes. in Liposome Technology Volume III: Targeted Drug Delivery and Biological Interaction. CRC Press, pp. 51-62. https://doi.org/10.1201/9781351074117
Huang L, Huang A, Kennel S. Coupling of antibodies with liposomes. In Liposome Technology Volume III: Targeted Drug Delivery and Biological Interaction. CRC Press. 2018. p. 51-62 https://doi.org/10.1201/9781351074117
Huang, Leaf ; Huang, Anthony ; Kennel, Stephen. / Coupling of antibodies with liposomes. Liposome Technology Volume III: Targeted Drug Delivery and Biological Interaction. CRC Press, 2018. pp. 51-62
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