CpG DNA activates c-jun NH2-terminal kinase

Ae-Kyung Yi, Demetrius P. Gravis, Arthur M. Krieg

Research output: Contribution to journalArticle

Abstract

Unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs) in bacterial DNA or synthetic oligonucleotides (CpG DNA) promote B cell proliferation and rescue B cells from apoptosis. CpG DNA also induces secretion of several pro-inflammatory cytokines. Recent studies have demonstrated that CpG DNA activates NFκB, which is required for induction of subsequent gene expression. Mitogenactivated protein kineses (MAPKs) are important mediators of the cellular stress or mitogenic response. Therefore, we investigated whether CpG DNA-induced B cell activation is mediated through activation of one or more members of the MAPK superfamily and whether NFκB activation by CpG DNA has an effect on MAPK activation. The present study demonstrated that CpG DNA, but not control non-CpG DNA, induced activation of c-jun NH2-terminal kinase (JNK). However, CpG DNA did not induce ERK activation in WEHI-231 B cells. CpG DNA-mediated JNK activation was abolished by chloroquine, an endosomal acidification inhibitor, but not by gliotoxin, an NFκB activation inhibitor. Our results suggest that activation of JNK by CpG DNA requires endosomal acidification but not NFκB activation.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

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JNK Mitogen-Activated Protein Kinases
mitogen-activated protein kinase
Chemical activation
DNA
B-lymphocytes
B-Lymphocytes
Acidification
Cells
acidification
Gliotoxin
oligodeoxyribonucleotides
Bacterial DNA
Proteins
chloroquine
Chloroquine
Cell proliferation
proteins
Gene expression
Oligonucleotides
cell proliferation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Yi, A-K., Gravis, D. P., & Krieg, A. M. (1998). CpG DNA activates c-jun NH2-terminal kinase. FASEB Journal, 12(5).

CpG DNA activates c-jun NH2-terminal kinase. / Yi, Ae-Kyung; Gravis, Demetrius P.; Krieg, Arthur M.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Yi, A-K, Gravis, DP & Krieg, AM 1998, 'CpG DNA activates c-jun NH2-terminal kinase', FASEB Journal, vol. 12, no. 5.
Yi A-K, Gravis DP, Krieg AM. CpG DNA activates c-jun NH2-terminal kinase. FASEB Journal. 1998 Mar 20;12(5).
Yi, Ae-Kyung ; Gravis, Demetrius P. ; Krieg, Arthur M. / CpG DNA activates c-jun NH2-terminal kinase. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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