Crocin has anti-inflammatory and protective effects in ischemia-reperfusion induced renal injuries

Zeynab Mohamadi Yarijani, Ali Pourmotabbed, Tayebeh Pourmotabbed, Houshang Najafi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective(s): Crocus sativus (saffron) has been widely used in traditional medicine. It has also been found to possess many beneficial properties in modern medicine. The most important ingredients of saffron are crocin, crocetin, safranal, and picrocrocin. This study evaluated the protective effects of crocin against the inflammation, oxidative stress, and functional disturbances of the kidney induced by renal ischemia/reperfusion (I/R). Materials and Methods: Different doses of crocin (0, 100, 200, and 400 mg/kg) were administered intraperitoneally 30 min before I/R. The rats of the sham group were also injected with normal saline before the sham surgery. For induction of I/R, both renal artery and vein clamped for 30 min, bilaterally. The I/R-induced renal injuries were assessed by measuring leukocyte infiltration, intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor-alpha (TNF-α) mRNA expression levels, malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP) levels in the kidney tissue, and plasma creatinine and urea-nitrogen concentrations. Results: Except for the tissue level of FRAP which decreased, all other measured parameters increased following I/R induction. Pretreatment with all doses of crocin significantly reduced the severity of these disturbances (P<0.05 to P<0.001). In fact, while there was no significant differences between MDA and FRAP levels, plasma creatinine and urea-nitrogen concentrations of the crocin-treated animals and the sham group, crocin administration reduced leukocyte infiltration and ICAM-1 and TNF-α mRNA expression levels in a dose-dependent manner. Conclusion: The present study clearly demonstrated the anti-inflammatory, antioxidant, and protective effects of crocin, a main constituent of saffron, against renal damages resulted from I/R in rats.

Original languageEnglish (US)
Pages (from-to)754-760
Number of pages7
JournalIranian Journal of Basic Medical Sciences
Volume20
Issue number7
DOIs
StatePublished - Jan 1 2017

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Reperfusion
Anti-Inflammatory Agents
Ischemia
Kidney
Wounds and Injuries
Antioxidants
Intercellular Adhesion Molecule-1
Malondialdehyde
Infiltration
Medicine
Urea
Rats
Creatinine
Nitrogen
Tumor Necrosis Factor-alpha
Tissue
Plasmas
Messenger RNA
Modern 1601-history
Renal Veins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Immunology and Microbiology(all)
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Crocin has anti-inflammatory and protective effects in ischemia-reperfusion induced renal injuries. / Mohamadi Yarijani, Zeynab; Pourmotabbed, Ali; Pourmotabbed, Tayebeh; Najafi, Houshang.

In: Iranian Journal of Basic Medical Sciences, Vol. 20, No. 7, 01.01.2017, p. 754-760.

Research output: Contribution to journalArticle

Mohamadi Yarijani, Zeynab ; Pourmotabbed, Ali ; Pourmotabbed, Tayebeh ; Najafi, Houshang. / Crocin has anti-inflammatory and protective effects in ischemia-reperfusion induced renal injuries. In: Iranian Journal of Basic Medical Sciences. 2017 ; Vol. 20, No. 7. pp. 754-760.
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abstract = "Objective(s): Crocus sativus (saffron) has been widely used in traditional medicine. It has also been found to possess many beneficial properties in modern medicine. The most important ingredients of saffron are crocin, crocetin, safranal, and picrocrocin. This study evaluated the protective effects of crocin against the inflammation, oxidative stress, and functional disturbances of the kidney induced by renal ischemia/reperfusion (I/R). Materials and Methods: Different doses of crocin (0, 100, 200, and 400 mg/kg) were administered intraperitoneally 30 min before I/R. The rats of the sham group were also injected with normal saline before the sham surgery. For induction of I/R, both renal artery and vein clamped for 30 min, bilaterally. The I/R-induced renal injuries were assessed by measuring leukocyte infiltration, intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor-alpha (TNF-α) mRNA expression levels, malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP) levels in the kidney tissue, and plasma creatinine and urea-nitrogen concentrations. Results: Except for the tissue level of FRAP which decreased, all other measured parameters increased following I/R induction. Pretreatment with all doses of crocin significantly reduced the severity of these disturbances (P<0.05 to P<0.001). In fact, while there was no significant differences between MDA and FRAP levels, plasma creatinine and urea-nitrogen concentrations of the crocin-treated animals and the sham group, crocin administration reduced leukocyte infiltration and ICAM-1 and TNF-α mRNA expression levels in a dose-dependent manner. Conclusion: The present study clearly demonstrated the anti-inflammatory, antioxidant, and protective effects of crocin, a main constituent of saffron, against renal damages resulted from I/R in rats.",
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AB - Objective(s): Crocus sativus (saffron) has been widely used in traditional medicine. It has also been found to possess many beneficial properties in modern medicine. The most important ingredients of saffron are crocin, crocetin, safranal, and picrocrocin. This study evaluated the protective effects of crocin against the inflammation, oxidative stress, and functional disturbances of the kidney induced by renal ischemia/reperfusion (I/R). Materials and Methods: Different doses of crocin (0, 100, 200, and 400 mg/kg) were administered intraperitoneally 30 min before I/R. The rats of the sham group were also injected with normal saline before the sham surgery. For induction of I/R, both renal artery and vein clamped for 30 min, bilaterally. The I/R-induced renal injuries were assessed by measuring leukocyte infiltration, intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor-alpha (TNF-α) mRNA expression levels, malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP) levels in the kidney tissue, and plasma creatinine and urea-nitrogen concentrations. Results: Except for the tissue level of FRAP which decreased, all other measured parameters increased following I/R induction. Pretreatment with all doses of crocin significantly reduced the severity of these disturbances (P<0.05 to P<0.001). In fact, while there was no significant differences between MDA and FRAP levels, plasma creatinine and urea-nitrogen concentrations of the crocin-treated animals and the sham group, crocin administration reduced leukocyte infiltration and ICAM-1 and TNF-α mRNA expression levels in a dose-dependent manner. Conclusion: The present study clearly demonstrated the anti-inflammatory, antioxidant, and protective effects of crocin, a main constituent of saffron, against renal damages resulted from I/R in rats.

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