Cross-reactive antibodies to target proteins are dependent upon oligomannose glycosylated epitopes in HTLV-1 associated neurological disease

Sang Lee, Yoojin Shin, Daniel Clark, Eduardo Gotuzzo, Michael C. Levin

Research output: Contribution to journalArticle

Abstract

Our lab recently identified a cross-reactive antibody response between human T-lymphotropic virus type-1- p24-(gag) (HTLV-1-p24-(gag)) and peroxiredoxin-1 (PrX- 1) as potentially contributing to the pathogenesis of HTLV-1 associated neurological disease via molecular mimicry. These targets proteins were glycosylated, yet the glycan side chains immunoreactive with the immunoglobulins were unknown. Using a combination of lectin isolation and serial enzymatic deglycosylation of glycoproteins, we determined that the immunoreactive epitopes contained branched oligomannose side chains. These data suggest that posttranslational glycosylation specifically related to oligomannose immunoreactivity to both the infecting and host antigens may contribute to molecular mimicry and be important in the pathogenesis of HTLV-1 associated neurological disease.

Original languageEnglish (US)
Pages (from-to)736-745
Number of pages10
JournalJournal of Clinical Immunology
Volume32
Issue number4
DOIs
StatePublished - Aug 1 2012

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Human T-lymphotropic virus 1
Epitopes
Molecular Mimicry
Antibodies
Proteins
Peroxiredoxins
Glycosylation
Lectins
Antibody Formation
Polysaccharides
Immunoglobulins
Glycoproteins
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Cross-reactive antibodies to target proteins are dependent upon oligomannose glycosylated epitopes in HTLV-1 associated neurological disease. / Lee, Sang; Shin, Yoojin; Clark, Daniel; Gotuzzo, Eduardo; Levin, Michael C.

In: Journal of Clinical Immunology, Vol. 32, No. 4, 01.08.2012, p. 736-745.

Research output: Contribution to journalArticle

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