Crystal structure of full-length KcsA in its closed conformation

Serdar Uysal, Valeria Vásquez, Valentina Tereshko, Kaori Esaki, Frederic A. Fellouse, Sachdev S. Sidhu, Shohei Koide, Eduardo Perozo, Anthony Kossiakoff

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

KcsA is a proton-activated, voltage-modulated Kchannel that has served as the archetype pore domain in the Kv channel superfamily. Here, we have used synthetic antigen-binding fragments (Fabs) as crystallographic chaperones to determine the structure of full-length KcsA at 3.8 Å, as well as that of its isolated C-terminal domain at 2.6 Å. The structure of the full-length KcsA-Fab complex reveals a well-defined, 4-helix bundle that projects ∼ 70 Å toward the cytoplasm. This bundle promotes a ∼ 15° bending in the inner bundle gate, tightening its diameter and shifting the narrowest point 2 turns of helix below. Functional analysis of the full-length KcsA-Fab complex suggests that the C-terminal bundle remains whole during gating. We suggest that this structure likely represents the physiologically relevant closed conformation of KcsA.

Original languageEnglish (US)
Pages (from-to)6644-6649
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number16
DOIs
StatePublished - Apr 21 2009

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Synthetic Vaccines
Protons
Cytoplasm

All Science Journal Classification (ASJC) codes

  • General

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Crystal structure of full-length KcsA in its closed conformation. / Uysal, Serdar; Vásquez, Valeria; Tereshko, Valentina; Esaki, Kaori; Fellouse, Frederic A.; Sidhu, Sachdev S.; Koide, Shohei; Perozo, Eduardo; Kossiakoff, Anthony.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 16, 21.04.2009, p. 6644-6649.

Research output: Contribution to journalArticle

Uysal, S, Vásquez, V, Tereshko, V, Esaki, K, Fellouse, FA, Sidhu, SS, Koide, S, Perozo, E & Kossiakoff, A 2009, 'Crystal structure of full-length KcsA in its closed conformation', Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 16, pp. 6644-6649. https://doi.org/10.1073/pnas.0810663106
Uysal, Serdar ; Vásquez, Valeria ; Tereshko, Valentina ; Esaki, Kaori ; Fellouse, Frederic A. ; Sidhu, Sachdev S. ; Koide, Shohei ; Perozo, Eduardo ; Kossiakoff, Anthony. / Crystal structure of full-length KcsA in its closed conformation. In: Proceedings of the National Academy of Sciences of the United States of America. 2009 ; Vol. 106, No. 16. pp. 6644-6649.
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AU - Vásquez, Valeria

AU - Tereshko, Valentina

AU - Esaki, Kaori

AU - Fellouse, Frederic A.

AU - Sidhu, Sachdev S.

AU - Koide, Shohei

AU - Perozo, Eduardo

AU - Kossiakoff, Anthony

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