Culturing and neuronal differentiation of human dental pulp stem cells

Sarita Goorha, Lawrence Reiter

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A major issue in studying human neurogenetic disorders, especially rare syndromes affecting the nervous system, is the ability to grow neuronal cultures that accurately represent these disorders for analysis. Although there has been some success in generating induced pluripotent stem (iPS) cells from both skin and blood, there are still limitations to the collection and production of iPS cells from these biospecimens. We have had significant success in collecting and growing human dental pulp stem (DPS) cells from exfoliated teeth sent to our laboratory by the parents of children with a variety of rare neurogenetic syndromes. This protocol outlines our current methods for the growth and expansion of DPS cells from exfoliated (baby) teeth. These DPS cells can be differentiated into a variety of cell types including osteoblasts, chondrocytes, and mixed neuron and glial cultures. Here we provide our protocol for the differentiation of early passage DPS cell cultures into neurons for molecular studies.

Original languageEnglish (US)
Pages (from-to)21.6.1-21.6.10
JournalCurrent Protocols in Human Genetics
Volume2017
DOIs
StatePublished - Jan 1 2017

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Dental Pulp
Stem Cells
Induced Pluripotent Stem Cells
Neurons
Aptitude
Deciduous Tooth
Chondrocytes
Osteoblasts
Neuroglia
Nervous System
Tooth
Cell Culture Techniques
Parents
Skin
Growth

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Culturing and neuronal differentiation of human dental pulp stem cells. / Goorha, Sarita; Reiter, Lawrence.

In: Current Protocols in Human Genetics, Vol. 2017, 01.01.2017, p. 21.6.1-21.6.10.

Research output: Contribution to journalArticle

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