Curcumin protects retinal cells from light-and oxidant stress-induced cell death

Nawajes Mandal, Jagan M.R. Patlolla, Lixin Zheng, Martin Paul Agbaga, Julie Thu A. Tran, Lea Wicker, Anne Kasus-Jacobi, Michael H. Elliott, Chinthalapally V. Rao, Robert E. Anderson

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Age-related macular degeneration (AMD) is a complex disease that has potential involvement of inflammatory and oxidative stress-related pathways in its pathogenesis. In search of effective therapeutic agents, we tested curcumin, a naturally occurring compound with known anti-inflammatory and antioxidative properties, in a rat model of light-induced retinal degeneration (LIRD) and in retina-derived cell lines. We hypothesized that any compound effective against LIRD, which involves significant oxidative stress and inflammation, would be a candidate for further characterization for its potential application in AMD. We observed significant retinal neuroprotection in rats fed diets supplemented with curcumin (0.2% in diet) for 2 weeks. The mechanism of retinal protection from LIRD by curcumin involves inhibition of NF-κB activation and down-regulation of cellular inflammatory genes. When tested on retina-derived cell lines (661W and ARPE-19), pretreatment of curcumin protected these cells from H2O2-induced cell death by up-regulating cellular protective enzymes, such as HO-1, thioredoxin. Since, curcumin with its pleiotropic activities can modulate the expression and activation of many cellular regulatory proteins such as NF-κB, AKT, NRF2, and growth factors, which in turn inhibit cellular inflammatory responses and protect cells; we speculate that curcumin would be an effective nutraceutical compound for preventive and augmentative therapy of AMD.

Original languageEnglish (US)
Pages (from-to)672-679
Number of pages8
JournalFree Radical Biology and Medicine
Volume46
Issue number5
DOIs
StatePublished - Mar 1 2009

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Curcumin
Cell death
Oxidants
Cell Death
Cells
Light
Retinal Degeneration
Macular Degeneration
Oxidative stress
Nutrition
Retina
Rats
Oxidative Stress
Chemical activation
Diet
Cell Line
Thioredoxins
Dietary Supplements
Intercellular Signaling Peptides and Proteins
Anti-Inflammatory Agents

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

Cite this

Mandal, N., Patlolla, J. M. R., Zheng, L., Agbaga, M. P., Tran, J. T. A., Wicker, L., ... Anderson, R. E. (2009). Curcumin protects retinal cells from light-and oxidant stress-induced cell death. Free Radical Biology and Medicine, 46(5), 672-679. https://doi.org/10.1016/j.freeradbiomed.2008.12.006

Curcumin protects retinal cells from light-and oxidant stress-induced cell death. / Mandal, Nawajes; Patlolla, Jagan M.R.; Zheng, Lixin; Agbaga, Martin Paul; Tran, Julie Thu A.; Wicker, Lea; Kasus-Jacobi, Anne; Elliott, Michael H.; Rao, Chinthalapally V.; Anderson, Robert E.

In: Free Radical Biology and Medicine, Vol. 46, No. 5, 01.03.2009, p. 672-679.

Research output: Contribution to journalArticle

Mandal, N, Patlolla, JMR, Zheng, L, Agbaga, MP, Tran, JTA, Wicker, L, Kasus-Jacobi, A, Elliott, MH, Rao, CV & Anderson, RE 2009, 'Curcumin protects retinal cells from light-and oxidant stress-induced cell death', Free Radical Biology and Medicine, vol. 46, no. 5, pp. 672-679. https://doi.org/10.1016/j.freeradbiomed.2008.12.006
Mandal, Nawajes ; Patlolla, Jagan M.R. ; Zheng, Lixin ; Agbaga, Martin Paul ; Tran, Julie Thu A. ; Wicker, Lea ; Kasus-Jacobi, Anne ; Elliott, Michael H. ; Rao, Chinthalapally V. ; Anderson, Robert E. / Curcumin protects retinal cells from light-and oxidant stress-induced cell death. In: Free Radical Biology and Medicine. 2009 ; Vol. 46, No. 5. pp. 672-679.
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