Curvature of mouse satellite DNA and condensation of heterochromatin

Marko Radic, Karen Lundgren, Barbara A. Hamkalo

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

Cloned, sequenced mouse satellite DNA exhibits properties characteristic of molecules that possess a stable curvature. Circularly permuted fragments containing the region predicted to bend were used to map the curvature relative to DNA sequence. The altered mobility of these fragments in polyacrylamide gels is reversed when gels are run in the presence of distamycin A, a drug that binds preferentially to AT-rich DNA. Treatment of living mouse cells with this drug dramatically reduces the condensation of centromeric heterochromatin, the exclusive location of satellite sequences. In situ hybridization of satellite probes to extended chromosomes at the electron microscope level shows that satellite does not comprise a single block but is distributed throughout the centromere region. Based on these experiments, we hypothesize that the structure of mouse satellite DNA is an important feature of centromeric heterochromatin condensation.

Original languageEnglish (US)
Pages (from-to)1101-1108
Number of pages8
JournalCell
Volume50
Issue number7
DOIs
StatePublished - Sep 25 1987

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Satellite DNA
Heterochromatin
Condensation
Satellites
Centromere
DNA sequences
Chromosomes
Pharmaceutical Preparations
In Situ Hybridization
Electron microscopes
Gels
Electrons
Molecules
DNA
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Curvature of mouse satellite DNA and condensation of heterochromatin. / Radic, Marko; Lundgren, Karen; Hamkalo, Barbara A.

In: Cell, Vol. 50, No. 7, 25.09.1987, p. 1101-1108.

Research output: Contribution to journalArticle

Radic, Marko ; Lundgren, Karen ; Hamkalo, Barbara A. / Curvature of mouse satellite DNA and condensation of heterochromatin. In: Cell. 1987 ; Vol. 50, No. 7. pp. 1101-1108.
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