Cutting edge

Broad expression of the FoxP3 locus in epithelial cells: a caution against early interpretation of fatal inflammatory diseases following in vivo depletion of FoxP3-expressing cells

Guoyun Chen, Chong Chen, Lizhong Wang, Xing Chang, Pan Zheng, Yang Liu

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Dogma that the regulatory T cell (Treg) prevents catastrophic autoimmunity throughout the lifespan relies on the assumption that the FoxP3 locus is transcribed exclusively in Treg. To test the assumption, we used the Rag2 -/- and the Rag2-/- mice with the Scurfy (sf) mutation (FoxP3sf/Y or FoxP3sf/sf ) to evaluate FoxP3 expression outside of the lymphoid system. Immunohistochemistry and real-time PCR revealed FoxP3 expression in breast epithelial cells, lung respiratory epithelial cells, and prostate epithelial cells, although not in liver, heart, and intestine. The specificity of the assays was confirmed, as the signals were ablated by the Scurfy mutation of the FoxP3 gene. Using mice with a green fluorescence protein open reading frame knocked into the 3' untranslated region of the FoxP3 locus, we showed that the locus is transcribed broadly in epithelial cells of multiple organs. These results refute an essential underlying assumption of the dogma and question the specificity of FoxP3-based Treg depletion. The Journal of Immunology

Original languageEnglish (US)
Pages (from-to)5163-5166
Number of pages4
JournalJournal of Immunology
Volume180
Issue number8
DOIs
StatePublished - Apr 15 2008

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Epithelial Cells
Mutation
3' Untranslated Regions
Regulatory T-Lymphocytes
Allergy and Immunology
Autoimmunity
Open Reading Frames
Intestines
Real-Time Polymerase Chain Reaction
Prostate
Breast
Fluorescence
Immunohistochemistry
Lung
Liver
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Cutting edge : Broad expression of the FoxP3 locus in epithelial cells: a caution against early interpretation of fatal inflammatory diseases following in vivo depletion of FoxP3-expressing cells. / Chen, Guoyun; Chen, Chong; Wang, Lizhong; Chang, Xing; Zheng, Pan; Liu, Yang.

In: Journal of Immunology, Vol. 180, No. 8, 15.04.2008, p. 5163-5166.

Research output: Contribution to journalArticle

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