CXCR4 regulates migration of lung alveolar epithelial cells through activation of Rac1 and matrix metalloproteinase-2

Manik C. Ghosh, Patrudu S. Makena, Vijay Gorantla, Scott Sinclair, Christopher M. Waters

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Restoration of the epithelial barrier following acute lung injury is critical for recovery of lung homeostasis. After injury, alveolar type II epithelial (ATII) cells spread and migrate to cover the denuded surface and, eventually, proliferate and differentiate into type I cells. The chemokine CXCL12, also known as stromal cell-derived factor 1α, has wellrecognized roles in organogenesis, hematopoiesis, and immune responses through its binding to the chemokine receptor CXCR4. While CXCL12/CXCR4 signaling is known to be important in immune cell migration, the role of this chemokine-receptor interaction has not been studied in alveolar epithelial repair mechanisms. In this study, we demonstrated that secretion of CXCL12 was increased in the bronchoalveolar lavage of rats ventilated with an injurious tidal volume (25 ml/kg). We also found that CXCL12 secretion was increased by primary rat ATII cells and a mouse alveolar epithelial (MLE12) cell line following scratch wounding and that both types of cells express CXCR4. CXCL12 significantly increased ATII cell migration in a scratch-wound assay. When we treated cells with a specific antagonist for CXCR4, AMD-3100, cell migration was significantly inhibited. Knockdown of CXCR4 by short hairpin RNA (shRNA) caused decreased cell migration compared with cells expressing a nonspecific shRNA. Treatment with AMD-3100 decreased matrix metalloproteinase- 14 expression, increased tissue inhibitor of metalloproteinase-3 expression, decreased matrix metalloproteinase-2 activity, and prevented CXCL12-induced Rac1 activation. Similar results were obtained with shRNA knockdown of CXCR4. These findings may help identify a therapeutic target for augmenting epithelial repair following acute lung injury.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume302
Issue number9
DOIs
StatePublished - May 1 2012

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Alveolar Epithelial Cells
Matrix Metalloproteinase 2
Cell Movement
Small Interfering RNA
Chemokine CXCL12
Lung
Acute Lung Injury
Chemokine Receptors
Tissue Inhibitor of Metalloproteinase-3
Matrix Metalloproteinase 14
Organogenesis
Tidal Volume
Hematopoiesis
Wounds and Injuries
Bronchoalveolar Lavage
Homeostasis
Cell Line
Therapeutics
JM 3100

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

CXCR4 regulates migration of lung alveolar epithelial cells through activation of Rac1 and matrix metalloproteinase-2. / Ghosh, Manik C.; Makena, Patrudu S.; Gorantla, Vijay; Sinclair, Scott; Waters, Christopher M.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 302, No. 9, 01.05.2012.

Research output: Contribution to journalArticle

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