Cyclic nucleotides and cerebrovascular tone in newborn pigs

Elena Parfenova, M. Shibata, S. Zuckerman, R. Mirro, Charles Leffler

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Relationships between cyclic nucleotides and cerebrovascular tone were investigated using closed cranial windows in anesthetized newborn pigs. Pial arteriolar diameter was monitored and cerebrospinal fluid (CSF) was collected from beneath the cranial window. Adenosine 3',5'-cyclic monophosphate (cAMP) and guanosine 3',5'-cyclic monophosphate (cGMP) concentrations in CSF were 1,690 ± 200 and 730 ± 40 fmol/ml, respectively. Topically applied isozyme- selective and nonselective inhibitors [3-isobutyl-1-methylxanthine (IBMX), theophylline, Ro 201724, dipyridamole, zaprinast, calmidazolium, and W-7] of cyclic nucleotide phosphodiesterases dilated pial arterioles with concomitant increases in cAMP and/or cGMP levels in CSF. Topical application of dibutyryl-cAMP and dibutyryl-cGMP also resulted in pial arteriolar dilation. Ten-minute hypercapnia, which results in pial arteriolar dilation, increased cAMP to 5,240 ± 900 and cGMP to 1,350 ± 200 fmol/ml. IBMX and zaprinast potentiated the increases in cAMP and cGMP as well as the cerebrovascular dilation in response to hypercapnia. These data suggest that cyclic nucleotides contribute to regulation of cerebral vascular tone during control conditions. Furthermore, cAMP and/or cGMP appears to be involved in arterial vasodilation in response to hypercapnia in newborn pigs.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume265
Issue number6 34-6
StatePublished - Dec 1 1993

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Cyclic Nucleotides
Cyclic GMP
Swine
Hypercapnia
Cerebrospinal Fluid
Dilatation
calmidazolium
4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone
1-Methyl-3-isobutylxanthine
Dipyridamole
Phosphoric Diester Hydrolases
Arterioles
Theophylline
Vasodilation
Cyclic AMP
Isoenzymes
Blood Vessels

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Cyclic nucleotides and cerebrovascular tone in newborn pigs. / Parfenova, Elena; Shibata, M.; Zuckerman, S.; Mirro, R.; Leffler, Charles.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 265, No. 6 34-6, 01.12.1993.

Research output: Contribution to journalArticle

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