Cytochrome P450 1D1: A novel CYP1A-related gene that is not transcriptionally activated by PCB126 or TCDD

J. V. Goldstone, M. E. Jönsson, L. Behrendt, B. R. Woodin, M. J. Jenny, David Nelson, J. J. Stegeman

Research output: Contribution to journalArticle

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Abstract

Enzymes in the cytochrome P450 1 family oxidize many common environmental toxicants. We identified a new CYP1, termed CYP1D1, in zebrafish. Phylogenetically, CYP1D1 is paralogous to CYP1A and the two share 45% amino acid identity and similar gene structure. In adult zebrafish, CYP1D1 is most highly expressed in liver and is relatively highly expressed in brain. CYP1D1 transcript levels were higher at 9 h post-fertilization than at later developmental times. Treatment of zebrafish with potent aryl hydrocarbon receptor (AHR) agonists (3,3′,4,4′,5-pentachlorobiphenyl or 2,3,7,8-tetrachlorodibenzo-p-dioxin) did not induce CYP1D1 transcript expression. Morpholino oligonucleotide knockdown of AHR2, which mediates induction of other CYP1s, did not affect CYP1D1 expression. Zebrafish CYP1D1 heterologously expressed in yeast exhibited ethoxyresorufin- and methoxyresorufin-O-dealkylase activities. Antibodies against a CYP1D1 peptide specifically detected a single electrophoretically-resolved protein band in zebrafish liver microsomes, distinct from CYP1A. CYP1D1 in zebrafish is a CYP1A-like gene that could have metabolic functions targeting endogenous compounds.

Original languageEnglish (US)
Pages (from-to)7-16
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume482
Issue number1-2
DOIs
StatePublished - Feb 1 2009

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Zebrafish
Liver
Cytochrome P-450 Enzyme System
Genes
Aryl Hydrocarbon Receptors
Morpholinos
Yeast
Brain
Amino Acids
Peptides
Antibodies
Enzymes
Proteins
Liver Microsomes
Fertilization
Polychlorinated Dibenzodioxins
Yeasts
1,4-dioxin
ethoxyresorufin

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Cytochrome P450 1D1 : A novel CYP1A-related gene that is not transcriptionally activated by PCB126 or TCDD. / Goldstone, J. V.; Jönsson, M. E.; Behrendt, L.; Woodin, B. R.; Jenny, M. J.; Nelson, David; Stegeman, J. J.

In: Archives of Biochemistry and Biophysics, Vol. 482, No. 1-2, 01.02.2009, p. 7-16.

Research output: Contribution to journalArticle

Goldstone, J. V. ; Jönsson, M. E. ; Behrendt, L. ; Woodin, B. R. ; Jenny, M. J. ; Nelson, David ; Stegeman, J. J. / Cytochrome P450 1D1 : A novel CYP1A-related gene that is not transcriptionally activated by PCB126 or TCDD. In: Archives of Biochemistry and Biophysics. 2009 ; Vol. 482, No. 1-2. pp. 7-16.
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