Cytochrome P450 complement (CYPome) of the avermectin-producer Streptomyces avermitilis and comparison to that of Streptomyces coelicolor A3(2)

David C. Lamb, Haruo Ikeda, David Nelson, Jun Ishikawa, Tove Skaug, Colin Jackson, Satoshi Omura, Michael R. Waterman, Steven L. Kelly

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The genus Streptomyces produces about two-thirds of naturally occurring antibiotics and a wide array of other secondary metabolites, including antihelminthic agents, antitumor agents, antifungal agents, and herbicides. The newly completed genome sequence of the avermectin-producing bacterium Streptomyces avermitilis contains 33 cytochromes P450 (CYPs), many more than the 18 observed in Streptomyces coelicolor A3(2). Some of the likely metabolic functions are reported together with their genomic location and bioinformatic analysis. Seven entirely new CYP families were found together with close homologues of some forms observed in S. coelicolor A3(2). The presence of unusual CYP forms associated with conservons is revealed and of these, CYP157 forms in both S. avermitilis and S. coelicolor A3(2) deviate from the previously accepted rule for an EXXR motif within the K-helix of CYPs. Amongst this range of CYPs are forms associated with avermectin, filipin, geosmin, and pentalenolactone biosynthesis as well as unknown pathways of secondary metabolism.

Original languageEnglish (US)
Pages (from-to)610-619
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume307
Issue number3
DOIs
StatePublished - Aug 1 2003

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Streptomyces coelicolor
Streptomyces
Cytochrome P-450 Enzyme System
Filipin
Secondary Metabolism
Antifungal Agents
Biosynthesis
Herbicides
Bioinformatics
Metabolites
Computational Biology
Antineoplastic Agents
Bacteria
Genes
avermectin
Genome
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cytochrome P450 complement (CYPome) of the avermectin-producer Streptomyces avermitilis and comparison to that of Streptomyces coelicolor A3(2). / Lamb, David C.; Ikeda, Haruo; Nelson, David; Ishikawa, Jun; Skaug, Tove; Jackson, Colin; Omura, Satoshi; Waterman, Michael R.; Kelly, Steven L.

In: Biochemical and Biophysical Research Communications, Vol. 307, No. 3, 01.08.2003, p. 610-619.

Research output: Contribution to journalArticle

Lamb, David C. ; Ikeda, Haruo ; Nelson, David ; Ishikawa, Jun ; Skaug, Tove ; Jackson, Colin ; Omura, Satoshi ; Waterman, Michael R. ; Kelly, Steven L. / Cytochrome P450 complement (CYPome) of the avermectin-producer Streptomyces avermitilis and comparison to that of Streptomyces coelicolor A3(2). In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 307, No. 3. pp. 610-619.
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