Cytochrome p450 monooxygenase CYP139 family involved in the synthesis of secondary metabolites in 824 mycobacterial species

Puleng Rosinah Syed, Wanping Chen, David Nelson, Abidemi Paul Kappo, Jae Hyuk Yu, Rajshekhar Karpoormath, Khajamohiddin Syed

Research output: Contribution to journalArticle

Abstract

Tuberculosis (TB) is one of the top infectious diseases causing numerous human deaths in the world. Despite enormous efforts, the physiology of the causative agent, Mycobacterium tuberculosis, is poorly understood. To contribute to better understanding the physiological capacity of these microbes, we have carried out extensive in silico analyses of the 1111 mycobacterial species genomes focusing on revealing the role of the orphan cytochrome P450 monooxygenase (CYP) CYP139 family. We have found that CYP139 members are present in 894 species belonging to three mycobacterial groups: M. tuberculosis complex (850-species), Mycobacterium avium complex (34-species), and non-tuberculosis mycobacteria (10-species), with all CYP139 members belonging to the subfamily “A”. CYP139 members have unique amino acid patterns at the CXG motif. Amino acid conservation analysis placed this family in the 8th among CYP families belonging to different biological domains and kingdoms. Biosynthetic gene cluster analyses have revealed that 92% of CYP139As might be associated with producing different secondary metabolites. Such enhanced secondary metabolic potentials with the involvement of CYP139A members might have provided mycobacterial species with advantageous traits in diverse niches competing with other microbial or viral agents, and might help these microbes infect hosts by interfering with the hosts’ metabolism and immune system.

Original languageEnglish (US)
Article number2690
JournalInternational journal of molecular sciences
Volume20
Issue number11
DOIs
StatePublished - Jun 1 2019

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tuberculosis
metabolites
cytochromes
Metabolites
Mixed Function Oxygenases
Mycobacterium tuberculosis
Cytochrome P-450 Enzyme System
Amino acids
Genes
microorganisms
Amino Acids
Mycobacterium avium Complex
Orphaned Children
amino acids
Immune system
Physiology
synthesis
Mycobacterium
Multigene Family
Metabolism

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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Cytochrome p450 monooxygenase CYP139 family involved in the synthesis of secondary metabolites in 824 mycobacterial species. / Syed, Puleng Rosinah; Chen, Wanping; Nelson, David; Kappo, Abidemi Paul; Yu, Jae Hyuk; Karpoormath, Rajshekhar; Syed, Khajamohiddin.

In: International journal of molecular sciences, Vol. 20, No. 11, 2690, 01.06.2019.

Research output: Contribution to journalArticle

Syed, Puleng Rosinah ; Chen, Wanping ; Nelson, David ; Kappo, Abidemi Paul ; Yu, Jae Hyuk ; Karpoormath, Rajshekhar ; Syed, Khajamohiddin. / Cytochrome p450 monooxygenase CYP139 family involved in the synthesis of secondary metabolites in 824 mycobacterial species. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 11.
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