Cytokine inducible matrix metalloproteinase expression in immortalized rat chondrocytes is independent of nitric oxide stimulation

Walter E. Horton, Imelda Udo, Patricia Precht, Richard Balakir, Karen Hasty

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The objective of this study was to determine if an immortalized mammalian chondrocyte cell line had a profile of matrix metalloproteinase (MMP) expression that was consistent with what has been reported for primary chondrocytes in vitro and in vivo. A combination of zymography, Western, and Northern analysis was used to examine the expression of MMPs that are relevant to cartilage degradation. Both interleukin-1β and tumor necrosis factor α induced a 4- to 9-fold increase in the level of MMP-9 expression in conditioned media, and a 17- to 24-fold increase in MMP-3 mRNA. Other compounds such as basic fibroblast growth factor and staurosporine each increased MMP-9 expression individually and potentiated the effects of the two cytokines. Transforming growth factor β had no positive or inhibitory effects. N-methyl arginine blocked the increase in nitric oxide observed following treatment with the cytokines but did not prevent the increased expression of MMPs. The pattern of metalloproteinase expression observed in IRC cells and the response to cytokines is very similar to what has been reported during the pathogenesis of osteoarthritis. The IRC cells should be useful as a model system to study basic mechanisms controlling chondrocyte MMP expression and to identify pharmacological modulators of this process.

Original languageEnglish (US)
Pages (from-to)378-384
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume34
Issue number5
DOIs
StatePublished - Jan 1 1998

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Chondrocytes
Matrix Metalloproteinases
Nitric Oxide
Cytokines
Matrix Metalloproteinase 9
Matrix Metalloproteinase 3
Staurosporine
Transforming Growth Factors
Metalloproteases
Fibroblast Growth Factor 2
Conditioned Culture Medium
Interleukin-1
Osteoarthritis
Cartilage
Arginine
Tumor Necrosis Factor-alpha
Cell Line
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Cytokine inducible matrix metalloproteinase expression in immortalized rat chondrocytes is independent of nitric oxide stimulation. / Horton, Walter E.; Udo, Imelda; Precht, Patricia; Balakir, Richard; Hasty, Karen.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 34, No. 5, 01.01.1998, p. 378-384.

Research output: Contribution to journalArticle

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