Cytoprotective role of vitamin E in porcine adipose-tissue-derived mesenchymal stem cells against hydrogen-peroxide-induced oxidative stress

Fazal Ur Rehman Bhatti, Song Ja Kim, Ae-Kyung Yi, Karen Hasty, Hongsik Cho

Research output: Contribution to journalArticle

Abstract

Survival of mesenchymal stem cells (MSCs) against oxidative stress and inflammation is vital for effective stem cell therapy. The reactive oxygen species (ROS) result in apoptosis and release of inflammatory mediators. Adipose-derived stem cells (ASCs) have shown promise for stem cell therapy owing to their anti-inflammatory and anti-oxidant activity. Previously, we showed the benefits of vitamin E against hydrogen peroxide (H2O2)-induced oxidative stress in rat bone marrow-derived MSCs. In this study, we aim to evaluate the effect of vitamin E treatment on porcine adipose-derived mesenchymal stem cells (pASCs) against H2O2-induced oxidative stress. The oxidative stress was induced by treating pASCs with 500 μM H2O2 with or without vitamin E. Viability of pASCs is enhanced after vitamin E treatment. In addition, reduced cellular toxicity, total NO level, PGE2 production and caspase-3 activity were observed after vitamin E treatment. Gene expression analysis of vitamin E-treated pASCs showed down-regulated expression for the genes associated with oxidative stress and apoptosis, viz., NOS2, Casp3, p53, BAX, MDM2, NFκB, HIF1α and VEGF-A genes. On the other hand, expression of anti-apoptotic and survival genes was up-regulated, viz., BCL2, BCL2L1 and MCL1. Furthermore, phosphorylation of Akt was attenuated following vitamin E treatment. The findings of this study may help in developing effective stem cell therapy for the diseases characterized by the oxidative stress and inflammation.

Original languageEnglish (US)
Pages (from-to)111-120
Number of pages10
JournalCell and Tissue Research
Volume374
Issue number1
DOIs
StatePublished - Oct 1 2018

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Mesenchymal Stromal Cells
Vitamin E
Hydrogen Peroxide
Adipose Tissue
Oxidative Stress
Swine
Stem Cells
Cell- and Tissue-Based Therapy
vif Genes
Inflammation
Gene Expression
Therapeutics
Dinoprostone
Oxidants
Caspase 3
Vascular Endothelial Growth Factor A
Reactive Oxygen Species
Anti-Inflammatory Agents
Bone Marrow
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Cytoprotective role of vitamin E in porcine adipose-tissue-derived mesenchymal stem cells against hydrogen-peroxide-induced oxidative stress. / Bhatti, Fazal Ur Rehman; Kim, Song Ja; Yi, Ae-Kyung; Hasty, Karen; Cho, Hongsik.

In: Cell and Tissue Research, Vol. 374, No. 1, 01.10.2018, p. 111-120.

Research output: Contribution to journalArticle

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