Cytotoxicity of dental resin composites

An in vitro evaluation

Pietro Ausiello, Angela Cassese, Claudia Miele, Francesco Beguinot, Franklin Garcia-Godoy, Bruno Di Jeso, Luca Ulianich

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Resin-based dental restorative materials release residual monomers that may affect the vitality of pulp cells. The purpose of this study was to evaluate the cytotoxic effect of two light-cured restorative materials with and without bis-GMA resin, respectively (Clearfil Majesty Posterior and Clearfil Majesty Flow) and a self-curing one (Clearfil DC Core Automix) when applied to the fibroblast cell line NIH-3T3. Samples of the materials were light-cured and placed directly in contact to cells for 24, 48, 72 and 96h. Cytotoxicity was evaluated by measuring cell death by flow cytometry, cell proliferation by proliferation curves analysis and morphological changes by optical microscopy analysis. All the composite materials tested caused a decrease in cell proliferation, albeit at different degrees. However, only Clearfil DC Core Automix induced cell death, very likely by increasing apoptosis. Morphological alteration of treated cells was also evident, particularly in the Clearfil DC Core Automix-treated cells. The different cytotoxic effects of dental composites should be considered when selecting an appropriate resin-based dental restorative material for operative restorations.

Original languageEnglish (US)
Pages (from-to)451-457
Number of pages7
JournalJournal of Applied Toxicology
Volume33
Issue number6
DOIs
StatePublished - Jun 1 2013

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Cytotoxicity
Dental materials
Cells
Cell proliferation
Cell death
Dental Materials
Resins
Bisphenol A-Glycidyl Methacrylate
Dental composites
Cell Death
Flow cytometry
Cell Proliferation
Fibroblasts
Light
Restoration
Pulp
Optical microscopy
Curing
Monomers
Apoptosis

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Ausiello, P., Cassese, A., Miele, C., Beguinot, F., Garcia-Godoy, F., Jeso, B. D., & Ulianich, L. (2013). Cytotoxicity of dental resin composites: An in vitro evaluation. Journal of Applied Toxicology, 33(6), 451-457. https://doi.org/10.1002/jat.1765

Cytotoxicity of dental resin composites : An in vitro evaluation. / Ausiello, Pietro; Cassese, Angela; Miele, Claudia; Beguinot, Francesco; Garcia-Godoy, Franklin; Jeso, Bruno Di; Ulianich, Luca.

In: Journal of Applied Toxicology, Vol. 33, No. 6, 01.06.2013, p. 451-457.

Research output: Contribution to journalArticle

Ausiello, P, Cassese, A, Miele, C, Beguinot, F, Garcia-Godoy, F, Jeso, BD & Ulianich, L 2013, 'Cytotoxicity of dental resin composites: An in vitro evaluation', Journal of Applied Toxicology, vol. 33, no. 6, pp. 451-457. https://doi.org/10.1002/jat.1765
Ausiello, Pietro ; Cassese, Angela ; Miele, Claudia ; Beguinot, Francesco ; Garcia-Godoy, Franklin ; Jeso, Bruno Di ; Ulianich, Luca. / Cytotoxicity of dental resin composites : An in vitro evaluation. In: Journal of Applied Toxicology. 2013 ; Vol. 33, No. 6. pp. 451-457.
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