Cytotoxicity of the glycolipid region of streptococcal lipoteichoic acid for cultures of human heart cells

W. Andrew Simpson, James Dale, Edwin H. Beachey

Research output: Contribution to journalArticle

Abstract

The ability of LTA of Streptococcus pyogenes to stimulate cell division or to kill tissue culture cells derived from human heart was investigated. Initial studies indicated that at low concentrations, ranging from 0.01 to 1.0 μg/ml, LTA stimulated cell division, whereas at higher concentrations, ranging from 10 to 1000 μg/ml, it killed the cells. Deacylated lipoteichoic acid, which lacked cell membrane binding activity, similarly stimulated or killed the heart cells depending on the concentration added to the tissue cultures. Fractionation of LTA after mild ammonia hydrolysis yielded a polyglycerophosphate and polar lipid fraction, both of which retained the glucose from the glycolipid moiety of the LTA molecule, and a neutral lipid fraction that was devoid of phosphorus or glucose. Toxic activity was present only in the fractions containing glucose. Oxidation of LTA or its deacylated derivative with sodium metaperiodate reduced the assayable glucose content without destroying the polyglycerophosphate backbone and resulted in a parallel loss of cytotoxicity, strongly suggesting that the glucose moieties of S. pyogenes LTA must be intact for the toxic activity against human heart cells to be expressed.

Original languageEnglish (US)
Pages (from-to)108-117
Number of pages10
JournalThe Journal of Laboratory and Clinical Medicine
Volume99
Issue number1
StatePublished - Jan 1 1982

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Glycolipids
Cytotoxicity
Glucose
Tissue culture
Poisons
Streptococcus pyogenes
Cell Division
Cells
Lipids
Cell membranes
Fractionation
Ammonia
Human Activities
Phosphorus
Hydrolysis
Cell Culture Techniques
Cell Membrane
lipoteichoic acid
Derivatives
Oxidation

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Cytotoxicity of the glycolipid region of streptococcal lipoteichoic acid for cultures of human heart cells. / Simpson, W. Andrew; Dale, James; Beachey, Edwin H.

In: The Journal of Laboratory and Clinical Medicine, Vol. 99, No. 1, 01.01.1982, p. 108-117.

Research output: Contribution to journalArticle

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