Data-independent proteome analysis of ARPE-19 cells

Research output: Contribution to journalArticle

Abstract

We have performed a proteomics analysis of a human retinal pigment epithelial cell line (ARPE-19), which represents a widely used model for in vitro studies of cellular and molecular mechanisms related to human RPE cells (Dunn et al., 1996; Weigel et al., 2002) [1,2]. Whole cell protein extracts were separated in four gel fractions via short (10 min) SDS-PAGE runs. Following fractionation and trypsin digestion, the resulting peptides were separated on a nano UPLC LC system and analyzed on-line with a QTof-IMS instrument: a tandem mass spectrometer with ion mobility separation (Synapt G2-Si). Data were acquired in data-independent mode (UDMSE), which allows for absolute and/or relative post-acquisition protein quantification (Silva et al., 2006) [3]. The proteome profile data obtained from this study can be used as a protein reference database with qualitative and quantitative protein information related to ARPE-19 cells under normal growth conditions.

Original languageEnglish (US)
Pages (from-to)333-336
Number of pages4
JournalData in Brief
Volume20
DOIs
StatePublished - Oct 1 2018

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Proteome
Online Systems
Protein Databases
Proteins
Retinal Pigments
Cell Extracts
Proteomics
Trypsin
Polyacrylamide Gel Electrophoresis
Digestion
Gels
Epithelial Cells
Ions
Cell Line
Peptides
Growth

All Science Journal Classification (ASJC) codes

  • General

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Data-independent proteome analysis of ARPE-19 cells. / Koirala, Diwa; Beranova, Sarka; Giorgianni, Francesco.

In: Data in Brief, Vol. 20, 01.10.2018, p. 333-336.

Research output: Contribution to journalArticle

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