Db / db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet

Susan J. Burke, Heidi M. Batdorf, David H. Burk, Robert C. Noland, Adrianna E. Eder, Matthew S. Boulos, Michael Karlstad, J. Jason Collier

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To understand features of human obesity and type 2 diabetes mellitus (T2D) that can be recapitulated in the mouse, we compared C57BL/6J mice fed a Western-style diet (WD) to weight-matched genetically obese leptin receptor-deficient mice (db/db). All mice were monitored for changes in body composition, glycemia, and total body mass. To objectively compare diet-induced and genetic models of obesity, tissue analyses were conducted using mice with similar body mass. We found that adipose tissue inflammation was present in both models of obesity. In addition, distinct alterations in metabolic flexibility were evident between WD-fed mice and db/db mice. Circulating insulin levels are elevated in each model of obesity, while glucagon was increased only in the db/db mice. Although both WD-fed and db/db mice exhibited adaptive increases in islet size, the db/db mice also displayed augmented islet expression of the dedifferentiation marker Aldh1a3 and reduced nuclear presence of the transcription factor Nkx6.1. Based on the collective results put forth herein, we conclude that db/db mice capture key features of human T2D that do not occur in WD-fed C57BL/6J mice of comparable body mass.

Original languageEnglish (US)
Article number8503754
JournalJournal of Diabetes Research
Volume2017
DOIs
StatePublished - Jan 1 2017

Fingerprint

Inbred C57BL Mouse
Type 2 Diabetes Mellitus
Weights and Measures
Diet
Obesity
Western Diet
Genetic Models
Body Composition
Glucagon
Adipose Tissue
Transcription Factors
Insulin
Inflammation

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Burke, S. J., Batdorf, H. M., Burk, D. H., Noland, R. C., Eder, A. E., Boulos, M. S., ... Jason Collier, J. (2017). Db / db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet. Journal of Diabetes Research, 2017, [8503754]. https://doi.org/10.1155/2017/8503754

Db / db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet. / Burke, Susan J.; Batdorf, Heidi M.; Burk, David H.; Noland, Robert C.; Eder, Adrianna E.; Boulos, Matthew S.; Karlstad, Michael; Jason Collier, J.

In: Journal of Diabetes Research, Vol. 2017, 8503754, 01.01.2017.

Research output: Contribution to journalArticle

Burke, Susan J. ; Batdorf, Heidi M. ; Burk, David H. ; Noland, Robert C. ; Eder, Adrianna E. ; Boulos, Matthew S. ; Karlstad, Michael ; Jason Collier, J. / Db / db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet. In: Journal of Diabetes Research. 2017 ; Vol. 2017.
@article{5ab3a07558124d85a397e1a8bce12ddf,
title = "Db / db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet",
abstract = "To understand features of human obesity and type 2 diabetes mellitus (T2D) that can be recapitulated in the mouse, we compared C57BL/6J mice fed a Western-style diet (WD) to weight-matched genetically obese leptin receptor-deficient mice (db/db). All mice were monitored for changes in body composition, glycemia, and total body mass. To objectively compare diet-induced and genetic models of obesity, tissue analyses were conducted using mice with similar body mass. We found that adipose tissue inflammation was present in both models of obesity. In addition, distinct alterations in metabolic flexibility were evident between WD-fed mice and db/db mice. Circulating insulin levels are elevated in each model of obesity, while glucagon was increased only in the db/db mice. Although both WD-fed and db/db mice exhibited adaptive increases in islet size, the db/db mice also displayed augmented islet expression of the dedifferentiation marker Aldh1a3 and reduced nuclear presence of the transcription factor Nkx6.1. Based on the collective results put forth herein, we conclude that db/db mice capture key features of human T2D that do not occur in WD-fed C57BL/6J mice of comparable body mass.",
author = "Burke, {Susan J.} and Batdorf, {Heidi M.} and Burk, {David H.} and Noland, {Robert C.} and Eder, {Adrianna E.} and Boulos, {Matthew S.} and Michael Karlstad and {Jason Collier}, J.",
year = "2017",
month = "1",
day = "1",
doi = "10.1155/2017/8503754",
language = "English (US)",
volume = "2017",
journal = "Journal of Diabetes Research",
issn = "2314-6745",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Db / db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet

AU - Burke, Susan J.

AU - Batdorf, Heidi M.

AU - Burk, David H.

AU - Noland, Robert C.

AU - Eder, Adrianna E.

AU - Boulos, Matthew S.

AU - Karlstad, Michael

AU - Jason Collier, J.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - To understand features of human obesity and type 2 diabetes mellitus (T2D) that can be recapitulated in the mouse, we compared C57BL/6J mice fed a Western-style diet (WD) to weight-matched genetically obese leptin receptor-deficient mice (db/db). All mice were monitored for changes in body composition, glycemia, and total body mass. To objectively compare diet-induced and genetic models of obesity, tissue analyses were conducted using mice with similar body mass. We found that adipose tissue inflammation was present in both models of obesity. In addition, distinct alterations in metabolic flexibility were evident between WD-fed mice and db/db mice. Circulating insulin levels are elevated in each model of obesity, while glucagon was increased only in the db/db mice. Although both WD-fed and db/db mice exhibited adaptive increases in islet size, the db/db mice also displayed augmented islet expression of the dedifferentiation marker Aldh1a3 and reduced nuclear presence of the transcription factor Nkx6.1. Based on the collective results put forth herein, we conclude that db/db mice capture key features of human T2D that do not occur in WD-fed C57BL/6J mice of comparable body mass.

AB - To understand features of human obesity and type 2 diabetes mellitus (T2D) that can be recapitulated in the mouse, we compared C57BL/6J mice fed a Western-style diet (WD) to weight-matched genetically obese leptin receptor-deficient mice (db/db). All mice were monitored for changes in body composition, glycemia, and total body mass. To objectively compare diet-induced and genetic models of obesity, tissue analyses were conducted using mice with similar body mass. We found that adipose tissue inflammation was present in both models of obesity. In addition, distinct alterations in metabolic flexibility were evident between WD-fed mice and db/db mice. Circulating insulin levels are elevated in each model of obesity, while glucagon was increased only in the db/db mice. Although both WD-fed and db/db mice exhibited adaptive increases in islet size, the db/db mice also displayed augmented islet expression of the dedifferentiation marker Aldh1a3 and reduced nuclear presence of the transcription factor Nkx6.1. Based on the collective results put forth herein, we conclude that db/db mice capture key features of human T2D that do not occur in WD-fed C57BL/6J mice of comparable body mass.

UR - http://www.scopus.com/inward/record.url?scp=85029726087&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029726087&partnerID=8YFLogxK

U2 - 10.1155/2017/8503754

DO - 10.1155/2017/8503754

M3 - Article

VL - 2017

JO - Journal of Diabetes Research

JF - Journal of Diabetes Research

SN - 2314-6745

M1 - 8503754

ER -