De-novo transcriptome sequencing of a normalized cDNA pool from influenza infected ferrets

Jeremy V. Camp, Thomas L. Svensson, Alexis McBrayer, Colleen Jonsson, Peter Liljeström, Carl E. Bruder

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The ferret is commonly used as a model for studies of infectious diseases. The genomic sequence of this animal model is not yet characterized, and only a limited number of fully annotated cDNAs are currently available in GenBank. The majority of genes involved in innate or adaptive immune response are still lacking, restricting molecular genetic analysis of host response in the ferret model. To enable de novo identification of transcriptionally active ferret genes in response to infection, we performed de-novo transcriptome sequencing of animals infected with H1N1 A/California/07/2009. We also included splenocytes induced with bacterial lipopolysaccharide to allow for identification of transcripts specifically induced by Gram-negative bacteria. We pooled and normalized the cDNA library in order to delimit the risk of sequencing only highly expressed genes. While normalization of the cDNA library removes the possibility of assessing expression changes between individual animals, it has been shown to increase identification of low abundant transcripts. In this study, we identified more than 19000 partial ferret transcripts, including more than 1000 gene orthologs known to be involved in the innate and the adaptive immune response.

Original languageEnglish (US)
Article numbere37104
JournalPLoS One
Volume7
Issue number5
DOIs
StatePublished - May 11 2012
Externally publishedYes

Fingerprint

Ferrets
ferrets
transcriptomics
Transcriptome
influenza
Human Influenza
Complementary DNA
Genes
Animals
Adaptive Immunity
Gene Library
Innate Immunity
cDNA libraries
genes
Nucleic Acid Databases
splenocytes
Gram-Negative Bacteria
Gram-negative bacteria
molecular genetics
lipopolysaccharides

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Camp, J. V., Svensson, T. L., McBrayer, A., Jonsson, C., Liljeström, P., & Bruder, C. E. (2012). De-novo transcriptome sequencing of a normalized cDNA pool from influenza infected ferrets. PLoS One, 7(5), [e37104]. https://doi.org/10.1371/journal.pone.0037104

De-novo transcriptome sequencing of a normalized cDNA pool from influenza infected ferrets. / Camp, Jeremy V.; Svensson, Thomas L.; McBrayer, Alexis; Jonsson, Colleen; Liljeström, Peter; Bruder, Carl E.

In: PLoS One, Vol. 7, No. 5, e37104, 11.05.2012.

Research output: Contribution to journalArticle

Camp, Jeremy V. ; Svensson, Thomas L. ; McBrayer, Alexis ; Jonsson, Colleen ; Liljeström, Peter ; Bruder, Carl E. / De-novo transcriptome sequencing of a normalized cDNA pool from influenza infected ferrets. In: PLoS One. 2012 ; Vol. 7, No. 5.
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