Decreased expression of tissue inhibitor of metalloproteinase 1 in stunned myocardium

Kourosh Baghelai, Ralph Marktanner, Jeffery Dattilo, Mary Peace M. Dattilo, Emma R. Jakoi, Dorne R. Yager, Raymond G. Makhoul, Andrew S. Wechsler

    Research output: Contribution to journalArticle

    27 Citations (Scopus)

    Abstract

    Ultrastructural studies of stunned myocardium have shown disorganization and loss of extracellular collagen and increased collagenase activity early after ischemia and reperfusion. The interplay between matrix metalloproteinase 1 (MMP-1) and tissue inhibitor of metalloproteinase 1 (TIMP-1) regulates the turnover of cardiac extracellular matrix fibrillar collagens. However, the gene expression of MMP-1 and TIMP-1 in stunned myocardium is not known. Here, we determined whether altered expression of MMP-1 and TIMP-1 occurs in globally stunned hearts. An isolated nonworking rabbit heart preparation, perfused with a bovine erythrocyte suspension in modified Krebs solution, was used. Two groups were studied: the stunned group was subjected to 20 min of normothermic global ischemia followed by 120 min of normal reperfusion (n = 8), and the control group underwent 140 min of uninterrupted perfusion (n = 7). The developed pressures at the end of reperfusion for ischemic and control hearts were 67.0 ± 2.73 and 83.1 ± 1.52 mm Hg (P < 0.006) respectively. Ribonuclease protection assays of total left ventricular RNA using riboprobes for MMP-1, TIMP-1, and 18S rRNA were performed. A significant decrease (twofold, P < 0.03) in TIMP-1 gene expression was found in the stunned hearts, while MMP-1 mRNA expression was unchanged. Thus, in early stunning, the decrease in TIMP-1 expression could tip the balance favoring enhanced metalloproteinase activity, promoting collagen turnover, and initiating extracellular matrix remodeling. This may contribute to delayed recovery from myocardial stunning.

    Original languageEnglish (US)
    Pages (from-to)35-39
    Number of pages5
    JournalJournal of Surgical Research
    Volume77
    Issue number1
    DOIs
    StatePublished - Jan 1 1998

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    Myocardial Stunning
    Tissue Inhibitor of Metalloproteinase-1
    Matrix Metalloproteinase 1
    Matrix Metalloproteinase Inhibitors
    Reperfusion
    Extracellular Matrix
    Collagen
    Ischemia
    Fibrillar Collagens
    Gene Expression
    Metalloproteases
    Collagenases
    Ribonucleases
    Suspensions
    Perfusion
    Erythrocytes
    RNA
    Rabbits
    Pressure
    Control Groups

    All Science Journal Classification (ASJC) codes

    • Surgery

    Cite this

    Baghelai, K., Marktanner, R., Dattilo, J., Dattilo, M. P. M., Jakoi, E. R., Yager, D. R., ... Wechsler, A. S. (1998). Decreased expression of tissue inhibitor of metalloproteinase 1 in stunned myocardium. Journal of Surgical Research, 77(1), 35-39. https://doi.org/10.1006/jsre.1998.5330

    Decreased expression of tissue inhibitor of metalloproteinase 1 in stunned myocardium. / Baghelai, Kourosh; Marktanner, Ralph; Dattilo, Jeffery; Dattilo, Mary Peace M.; Jakoi, Emma R.; Yager, Dorne R.; Makhoul, Raymond G.; Wechsler, Andrew S.

    In: Journal of Surgical Research, Vol. 77, No. 1, 01.01.1998, p. 35-39.

    Research output: Contribution to journalArticle

    Baghelai, K, Marktanner, R, Dattilo, J, Dattilo, MPM, Jakoi, ER, Yager, DR, Makhoul, RG & Wechsler, AS 1998, 'Decreased expression of tissue inhibitor of metalloproteinase 1 in stunned myocardium', Journal of Surgical Research, vol. 77, no. 1, pp. 35-39. https://doi.org/10.1006/jsre.1998.5330
    Baghelai K, Marktanner R, Dattilo J, Dattilo MPM, Jakoi ER, Yager DR et al. Decreased expression of tissue inhibitor of metalloproteinase 1 in stunned myocardium. Journal of Surgical Research. 1998 Jan 1;77(1):35-39. https://doi.org/10.1006/jsre.1998.5330
    Baghelai, Kourosh ; Marktanner, Ralph ; Dattilo, Jeffery ; Dattilo, Mary Peace M. ; Jakoi, Emma R. ; Yager, Dorne R. ; Makhoul, Raymond G. ; Wechsler, Andrew S. / Decreased expression of tissue inhibitor of metalloproteinase 1 in stunned myocardium. In: Journal of Surgical Research. 1998 ; Vol. 77, No. 1. pp. 35-39.
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