Deep sequencing of RSV from an adult challenge study and from naturally infected infants reveals heterogeneous diversification dynamics

Jessica W. Lau, Young In Kim Hoehamer, Ryan Murphy, Ruchi Newman, Xiao Yang, Michael Zody, John Devincenzo, Yonatan H. Grad

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

As RNA virus mutation occurs during replication within host cells, we hypothesized that viral evolution during acute infections in healthy hosts reflects host immune pressure. We therefore investigated the within-host diversification of human respiratory syncytial virus (RSV), a highly prevalent cause of acute respiratory infections. We evaluated healthy adults experimentally infected with an identical inoculum and infants hospitalized with naturally acquired infections. In aggregate, viral diversification in adults peaked at day 3, with overrepresentation of diversity in the matrix protein 2 (M2) and non-structural protein 2 (NS2) genes. In one subject, delayed viral clearance was accompanied by a late peak of diversity at day 10 in known and predicted B and T cell epitopes. In contrast, infant infections showed much less viral diversity. Our findings suggest multiple overlapping mechanisms for early control of acute viral infections, which may differ between age groups and host immune responses.

Original languageEnglish (US)
Pages (from-to)289-296
Number of pages8
JournalVirology
Volume510
DOIs
StatePublished - Oct 1 2017

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High-Throughput Nucleotide Sequencing
Respiratory Syncytial Viruses
Infection
Human respiratory syncytial virus
B-Lymphocyte Epitopes
T-Lymphocyte Epitopes
RNA Viruses
Virus Diseases
Respiratory Tract Infections
Proteins
Age Groups
Pressure
Mutation

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Deep sequencing of RSV from an adult challenge study and from naturally infected infants reveals heterogeneous diversification dynamics. / Lau, Jessica W.; Kim Hoehamer, Young In; Murphy, Ryan; Newman, Ruchi; Yang, Xiao; Zody, Michael; Devincenzo, John; Grad, Yonatan H.

In: Virology, Vol. 510, 01.10.2017, p. 289-296.

Research output: Contribution to journalArticle

Lau, Jessica W. ; Kim Hoehamer, Young In ; Murphy, Ryan ; Newman, Ruchi ; Yang, Xiao ; Zody, Michael ; Devincenzo, John ; Grad, Yonatan H. / Deep sequencing of RSV from an adult challenge study and from naturally infected infants reveals heterogeneous diversification dynamics. In: Virology. 2017 ; Vol. 510. pp. 289-296.
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