Dental mesenchymal stem cells

Dental pulp stem cells, periodontal ligament stem cells, apical papilla stem cells, and primary teeth stem cells—isolation, characterization, and expansion for tissue engineering

Mey Al-Habib, George Huang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Dental stem cells (DSCs) have been shown to possess great potential for multiple biomedical applications, especially for dental tissue regeneration. They are a special type of subpopulation of mesenchymal stem/stromal cells (MSCs) and present subtle differences from other types of MSCs. Therefore, it requires a specialized expertise to isolate, culture, and characterize these cells in vitro and in vivo. The purpose of this chapter is to share our experience in studying these cells. We will describe in detail laboratory protocols outlining how the cells are isolated, cultured, expanded, and characterized using various in vitro cellular and biochemical analyses, as well as an in vivo study model using immunocompromised mice to observe tissue regeneration after transplantation of these DSCs.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages59-76
Number of pages18
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1922
ISSN (Print)1064-3745

Fingerprint

Dental Pulp
Periodontal Ligament
Deciduous Tooth
Tissue Engineering
Mesenchymal Stromal Cells
Tooth
Stem Cells
Regeneration
Stem Cell Transplantation
Cultured Cells
Cell Culture Techniques
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Dental mesenchymal stem cells : Dental pulp stem cells, periodontal ligament stem cells, apical papilla stem cells, and primary teeth stem cells—isolation, characterization, and expansion for tissue engineering. / Al-Habib, Mey; Huang, George.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 59-76 (Methods in Molecular Biology; Vol. 1922).

Research output: Chapter in Book/Report/Conference proceedingChapter

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