Dental Pulp Stem Cells Model Early Life and Imprinted DNA Methylation Patterns

Keith Dunaway, Sarita Goorha, Lauren Matelski, Nora Urraca, Pamela J. Lein, Ian Korf, Lawrence Reiter, Janine M. LaSalle

Research output: Contribution to journalArticle

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Abstract

Early embryonic stages of pluripotency are modeled for epigenomic studies primarily with human embryonic stem cells (ESC) or induced pluripotent stem cells (iPSCs). For analysis of DNA methylation however, ESCs and iPSCs do not accurately reflect the DNA methylation levels found in preimplantation embryos. Whole genome bisulfite sequencing (WGBS) approaches have revealed the presence of large partially methylated domains (PMDs) covering 30%-40% of the genome in oocytes, preimplantation embryos, and placenta. In contrast, ESCs and iPSCs show abnormally high levels of DNA methylation compared to inner cell mass (ICM) or placenta. Here we show that dental pulp stem cells (DPSCs), derived from baby teeth and cultured in serum-containing media, have PMDs and mimic the ICM and placental methylome more closely than iPSCs and ESCs. By principal component analysis, DPSC methylation patterns were more similar to two other neural stem cell types of human derivation (EPI-NCSC and LUHMES) and placenta than were iPSCs, ESCs or other human cell lines (SH-SY5Y, B lymphoblast, IMR90). To test the suitability of DPSCs in modeling epigenetic differences associated with disease, we compared methylation patterns of DPSCs derived from children with chromosome 15q11.2-q13.3 maternal duplication (Dup15q) to controls. Differential methylation region (DMR) analyses revealed the expected Dup15q hypermethylation at the imprinting control region, as well as hypomethylation over SNORD116, and novel DMRs over 147 genes, including several autism candidate genes. Together these data suggest that DPSCs are a useful model for epigenomic and functional studies of human neurodevelopmental disorders. Stem Cells 2017;35:981–988.

Original languageEnglish (US)
Pages (from-to)981-988
Number of pages8
JournalStem Cells
Volume35
Issue number4
DOIs
StatePublished - Apr 1 2017

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Dental Pulp
Induced Pluripotent Stem Cells
DNA Methylation
Stem Cells
Epigenomics
Placenta
Methylation
Blastocyst
Dental Pulp Test
Genome
Deciduous Tooth
Neural Stem Cells
Autistic Disorder
Principal Component Analysis
Genes
Oocytes
Chromosomes
Mothers
Cell Line
Serum

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Dunaway, K., Goorha, S., Matelski, L., Urraca, N., Lein, P. J., Korf, I., ... LaSalle, J. M. (2017). Dental Pulp Stem Cells Model Early Life and Imprinted DNA Methylation Patterns. Stem Cells, 35(4), 981-988. https://doi.org/10.1002/stem.2563

Dental Pulp Stem Cells Model Early Life and Imprinted DNA Methylation Patterns. / Dunaway, Keith; Goorha, Sarita; Matelski, Lauren; Urraca, Nora; Lein, Pamela J.; Korf, Ian; Reiter, Lawrence; LaSalle, Janine M.

In: Stem Cells, Vol. 35, No. 4, 01.04.2017, p. 981-988.

Research output: Contribution to journalArticle

Dunaway, K, Goorha, S, Matelski, L, Urraca, N, Lein, PJ, Korf, I, Reiter, L & LaSalle, JM 2017, 'Dental Pulp Stem Cells Model Early Life and Imprinted DNA Methylation Patterns', Stem Cells, vol. 35, no. 4, pp. 981-988. https://doi.org/10.1002/stem.2563
Dunaway K, Goorha S, Matelski L, Urraca N, Lein PJ, Korf I et al. Dental Pulp Stem Cells Model Early Life and Imprinted DNA Methylation Patterns. Stem Cells. 2017 Apr 1;35(4):981-988. https://doi.org/10.1002/stem.2563
Dunaway, Keith ; Goorha, Sarita ; Matelski, Lauren ; Urraca, Nora ; Lein, Pamela J. ; Korf, Ian ; Reiter, Lawrence ; LaSalle, Janine M. / Dental Pulp Stem Cells Model Early Life and Imprinted DNA Methylation Patterns. In: Stem Cells. 2017 ; Vol. 35, No. 4. pp. 981-988.
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