Design and validation of a cyclic strain bioreactor to condition spatially-selective scaffolds in dual strain regimes

J. Matthew Goodhart, Jared O. Cooper, Richard Smith, John L. Williams, Warren O. Haggard, Joel D. Bumgardner

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The objective of this study was to design and validate a unique bioreactor design for applying spatially selective, linear, cyclic strain to degradable and non-degradable polymeric fabric scaffolds. This system uses a novel three-clamp design to apply cyclic strain via a computer controlled linear actuator to a specified zone of a scaffold while isolating the remainder of the scaffold from strain. Image analysis of polyethylene terephthalate (PET) woven scaffolds subjected to a 3% mechanical stretch demonstrated that the stretched portion of the scaffold experienced 2.97% ± 0.13% strain (mean ± standard deviation) while the unstretched portion experienced 0.02% ± 0.18% strain. NIH-3T3 fibroblast cells were cultured on the PET scaffolds and half of each scaffold was stretched 5% at 0.5 Hz for one hour per day for 14 days in the bioreactor. Cells were checked for viability and proliferation at the end of the 14 day period and levels of glycosaminoglycan (GAG) and collagen (hydroxyproline) were measured as indicators of extracellular matrix production. Scaffolds in the bioreactor showed a seven-fold increase in cell number over scaffolds cultured statically in tissue culture plastic petri dishes (control). Bioreactor scaffolds showed a lower concentration of GAG deposition per cell as compared to the control scaffolds largely due to the great increase in cell number. A 75% increase in hydroxyproline concentration per cell was seen in the bioreactor stretched scaffolds as compared to the control scaffolds. Surprisingly, little differences were experienced between the stretched and unstretched portions of the scaffolds for this study. This was largely attributed to the conditioned and shared media effect. Results indicate that the bioreactor system is capable of applying spatially-selective, linear, cyclic strain to cells growing on polymeric fabric scaffolds and evaluating the cellular and matrix responses to the applied strains.

Original languageEnglish (US)
Pages (from-to)345-360
Number of pages16
JournalProcesses
Volume2
Issue number2
DOIs
StatePublished - Jun 1 2014

Fingerprint

Bioreactors
Scaffolds
Hydroxyproline
Polyethylene Terephthalates
Glycosaminoglycans
Polyethylene terephthalates
Linear actuators
Tissue culture
Clamping devices
Fibroblasts
Scaffolds (biology)
Collagen
Image analysis
Cells

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Process Chemistry and Technology

Cite this

Design and validation of a cyclic strain bioreactor to condition spatially-selective scaffolds in dual strain regimes. / Goodhart, J. Matthew; Cooper, Jared O.; Smith, Richard; Williams, John L.; Haggard, Warren O.; Bumgardner, Joel D.

In: Processes, Vol. 2, No. 2, 01.06.2014, p. 345-360.

Research output: Contribution to journalArticle

Goodhart, J. Matthew ; Cooper, Jared O. ; Smith, Richard ; Williams, John L. ; Haggard, Warren O. ; Bumgardner, Joel D. / Design and validation of a cyclic strain bioreactor to condition spatially-selective scaffolds in dual strain regimes. In: Processes. 2014 ; Vol. 2, No. 2. pp. 345-360.
@article{3dbdd0c2275f493f87e2c06bc4dde4a8,
title = "Design and validation of a cyclic strain bioreactor to condition spatially-selective scaffolds in dual strain regimes",
abstract = "The objective of this study was to design and validate a unique bioreactor design for applying spatially selective, linear, cyclic strain to degradable and non-degradable polymeric fabric scaffolds. This system uses a novel three-clamp design to apply cyclic strain via a computer controlled linear actuator to a specified zone of a scaffold while isolating the remainder of the scaffold from strain. Image analysis of polyethylene terephthalate (PET) woven scaffolds subjected to a 3{\%} mechanical stretch demonstrated that the stretched portion of the scaffold experienced 2.97{\%} ± 0.13{\%} strain (mean ± standard deviation) while the unstretched portion experienced 0.02{\%} ± 0.18{\%} strain. NIH-3T3 fibroblast cells were cultured on the PET scaffolds and half of each scaffold was stretched 5{\%} at 0.5 Hz for one hour per day for 14 days in the bioreactor. Cells were checked for viability and proliferation at the end of the 14 day period and levels of glycosaminoglycan (GAG) and collagen (hydroxyproline) were measured as indicators of extracellular matrix production. Scaffolds in the bioreactor showed a seven-fold increase in cell number over scaffolds cultured statically in tissue culture plastic petri dishes (control). Bioreactor scaffolds showed a lower concentration of GAG deposition per cell as compared to the control scaffolds largely due to the great increase in cell number. A 75{\%} increase in hydroxyproline concentration per cell was seen in the bioreactor stretched scaffolds as compared to the control scaffolds. Surprisingly, little differences were experienced between the stretched and unstretched portions of the scaffolds for this study. This was largely attributed to the conditioned and shared media effect. Results indicate that the bioreactor system is capable of applying spatially-selective, linear, cyclic strain to cells growing on polymeric fabric scaffolds and evaluating the cellular and matrix responses to the applied strains.",
author = "Goodhart, {J. Matthew} and Cooper, {Jared O.} and Richard Smith and Williams, {John L.} and Haggard, {Warren O.} and Bumgardner, {Joel D.}",
year = "2014",
month = "6",
day = "1",
doi = "10.3390/pr2020345",
language = "English (US)",
volume = "2",
pages = "345--360",
journal = "Processes",
issn = "2227-9717",
publisher = "MDPI AG",
number = "2",

}

TY - JOUR

T1 - Design and validation of a cyclic strain bioreactor to condition spatially-selective scaffolds in dual strain regimes

AU - Goodhart, J. Matthew

AU - Cooper, Jared O.

AU - Smith, Richard

AU - Williams, John L.

AU - Haggard, Warren O.

AU - Bumgardner, Joel D.

PY - 2014/6/1

Y1 - 2014/6/1

N2 - The objective of this study was to design and validate a unique bioreactor design for applying spatially selective, linear, cyclic strain to degradable and non-degradable polymeric fabric scaffolds. This system uses a novel three-clamp design to apply cyclic strain via a computer controlled linear actuator to a specified zone of a scaffold while isolating the remainder of the scaffold from strain. Image analysis of polyethylene terephthalate (PET) woven scaffolds subjected to a 3% mechanical stretch demonstrated that the stretched portion of the scaffold experienced 2.97% ± 0.13% strain (mean ± standard deviation) while the unstretched portion experienced 0.02% ± 0.18% strain. NIH-3T3 fibroblast cells were cultured on the PET scaffolds and half of each scaffold was stretched 5% at 0.5 Hz for one hour per day for 14 days in the bioreactor. Cells were checked for viability and proliferation at the end of the 14 day period and levels of glycosaminoglycan (GAG) and collagen (hydroxyproline) were measured as indicators of extracellular matrix production. Scaffolds in the bioreactor showed a seven-fold increase in cell number over scaffolds cultured statically in tissue culture plastic petri dishes (control). Bioreactor scaffolds showed a lower concentration of GAG deposition per cell as compared to the control scaffolds largely due to the great increase in cell number. A 75% increase in hydroxyproline concentration per cell was seen in the bioreactor stretched scaffolds as compared to the control scaffolds. Surprisingly, little differences were experienced between the stretched and unstretched portions of the scaffolds for this study. This was largely attributed to the conditioned and shared media effect. Results indicate that the bioreactor system is capable of applying spatially-selective, linear, cyclic strain to cells growing on polymeric fabric scaffolds and evaluating the cellular and matrix responses to the applied strains.

AB - The objective of this study was to design and validate a unique bioreactor design for applying spatially selective, linear, cyclic strain to degradable and non-degradable polymeric fabric scaffolds. This system uses a novel three-clamp design to apply cyclic strain via a computer controlled linear actuator to a specified zone of a scaffold while isolating the remainder of the scaffold from strain. Image analysis of polyethylene terephthalate (PET) woven scaffolds subjected to a 3% mechanical stretch demonstrated that the stretched portion of the scaffold experienced 2.97% ± 0.13% strain (mean ± standard deviation) while the unstretched portion experienced 0.02% ± 0.18% strain. NIH-3T3 fibroblast cells were cultured on the PET scaffolds and half of each scaffold was stretched 5% at 0.5 Hz for one hour per day for 14 days in the bioreactor. Cells were checked for viability and proliferation at the end of the 14 day period and levels of glycosaminoglycan (GAG) and collagen (hydroxyproline) were measured as indicators of extracellular matrix production. Scaffolds in the bioreactor showed a seven-fold increase in cell number over scaffolds cultured statically in tissue culture plastic petri dishes (control). Bioreactor scaffolds showed a lower concentration of GAG deposition per cell as compared to the control scaffolds largely due to the great increase in cell number. A 75% increase in hydroxyproline concentration per cell was seen in the bioreactor stretched scaffolds as compared to the control scaffolds. Surprisingly, little differences were experienced between the stretched and unstretched portions of the scaffolds for this study. This was largely attributed to the conditioned and shared media effect. Results indicate that the bioreactor system is capable of applying spatially-selective, linear, cyclic strain to cells growing on polymeric fabric scaffolds and evaluating the cellular and matrix responses to the applied strains.

UR - http://www.scopus.com/inward/record.url?scp=85010662581&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85010662581&partnerID=8YFLogxK

U2 - 10.3390/pr2020345

DO - 10.3390/pr2020345

M3 - Article

VL - 2

SP - 345

EP - 360

JO - Processes

JF - Processes

SN - 2227-9717

IS - 2

ER -