Detection of bacterial infection with a fiber optic microendoscope

Nooman Mufti, Ying Kong, Jeffrey D. Cirillo, Kristen C. Maitland

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present the use of fiber optic microendoscopy to image bacterial infection in the skin and lungs using an animal model. The contact probe microendoscope we have constructed has a 4 μm resolution, a 750 μm field of view, and a 1 mm outer diameter. Well resolved regions of bacterial infection were imaged for subcutaneous inocula of 10 6 to 10 1 CFU and intra-tracheal inocula of 10 8 to 10 6 CFU. Results reveal a linear relationship between average fluorescence and CFU, suggesting potential for using this device for quantitative analysis. Detection limits of 10 4 CFU for skin samples and 10 7 CFU for lung tissue were determined. In addition, bacteria were also qualitatively visible in lung tissue down to 10 6 CFU. Confocal imaging was used to confirm the presence of bacteria in tissue samples.

Original languageEnglish (US)
Title of host publicationMedical Laser Applications and Laser-Tissue Interactions V
Volume8092
DOIs
StatePublished - Aug 1 2011
Externally publishedYes
EventMedical Laser Applications and Laser-Tissue Interactions V - Munich, Germany
Duration: May 24 2011May 26 2011

Other

OtherMedical Laser Applications and Laser-Tissue Interactions V
CountryGermany
CityMunich
Period5/24/115/26/11

Fingerprint

infectious diseases
inoculum
Bacterial Infections
lungs
Fiber optics
fiber optics
Tissue
Lung
bacteria
Bacteria
Skin
animal models
quantitative analysis
field of view
Limit of Detection
Animals
Animal Models
Fluorescence
Imaging techniques
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Mufti, N., Kong, Y., Cirillo, J. D., & Maitland, K. C. (2011). Detection of bacterial infection with a fiber optic microendoscope. In Medical Laser Applications and Laser-Tissue Interactions V (Vol. 8092). [80920A] https://doi.org/10.1117/12.896553

Detection of bacterial infection with a fiber optic microendoscope. / Mufti, Nooman; Kong, Ying; Cirillo, Jeffrey D.; Maitland, Kristen C.

Medical Laser Applications and Laser-Tissue Interactions V. Vol. 8092 2011. 80920A.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mufti, N, Kong, Y, Cirillo, JD & Maitland, KC 2011, Detection of bacterial infection with a fiber optic microendoscope. in Medical Laser Applications and Laser-Tissue Interactions V. vol. 8092, 80920A, Medical Laser Applications and Laser-Tissue Interactions V, Munich, Germany, 5/24/11. https://doi.org/10.1117/12.896553
Mufti N, Kong Y, Cirillo JD, Maitland KC. Detection of bacterial infection with a fiber optic microendoscope. In Medical Laser Applications and Laser-Tissue Interactions V. Vol. 8092. 2011. 80920A https://doi.org/10.1117/12.896553
Mufti, Nooman ; Kong, Ying ; Cirillo, Jeffrey D. ; Maitland, Kristen C. / Detection of bacterial infection with a fiber optic microendoscope. Medical Laser Applications and Laser-Tissue Interactions V. Vol. 8092 2011.
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