Detection of bacterial infection with a fiber optic microendoscope

Nooman Mufti, Ying Kong, Jeffrey D. Cirillo, Kristen C. Maitland

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present the use of fiber optic microendoscopy to image bacterial infection in the skin and lungs using an animal model. The contact probe microendoscope we have constructed has a 4 μm resolution, a 750 μm field of view, and a 1 mm outer diameter. Well resolved regions of bacterial infection were imaged for subcutaneous inocula of 106 to 101 CFU and intra-tracheal inocula of 108 to 106 CFU. Results reveal a linear relationship between average fluorescence and CFU, suggesting potential for using this device for quantitative analysis. Detection limits of 104 CFU for skin samples and 107 CFU for lung tissue were determined. In addition, bacteria were also qualitatively visible in lung tissue down to 106 CFU. Confocal imaging was used to confirm the presence of bacteria in tissue samples.

Original languageEnglish (US)
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2011
StatePublished - 2011
Externally publishedYes
EventEuropean Conference on Biomedical Optics, ECBO 2011 - Munich, Germany
Duration: May 22 2011May 26 2011

Other

OtherEuropean Conference on Biomedical Optics, ECBO 2011
CountryGermany
CityMunich
Period5/22/115/26/11

Fingerprint

infectious diseases
inoculum
lungs
Fiber optics
fiber optics
Tissue
bacteria
Bacteria
Skin
animal models
quantitative analysis
field of view
Animals
Fluorescence
Imaging techniques
fluorescence
probes
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Mufti, N., Kong, Y., Cirillo, J. D., & Maitland, K. C. (2011). Detection of bacterial infection with a fiber optic microendoscope. In European Conference on Biomedical Optics, ECBO 2011 [80920A]

Detection of bacterial infection with a fiber optic microendoscope. / Mufti, Nooman; Kong, Ying; Cirillo, Jeffrey D.; Maitland, Kristen C.

European Conference on Biomedical Optics, ECBO 2011. 2011. 80920A.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mufti, N, Kong, Y, Cirillo, JD & Maitland, KC 2011, Detection of bacterial infection with a fiber optic microendoscope. in European Conference on Biomedical Optics, ECBO 2011., 80920A, European Conference on Biomedical Optics, ECBO 2011, Munich, Germany, 5/22/11.
Mufti N, Kong Y, Cirillo JD, Maitland KC. Detection of bacterial infection with a fiber optic microendoscope. In European Conference on Biomedical Optics, ECBO 2011. 2011. 80920A
Mufti, Nooman ; Kong, Ying ; Cirillo, Jeffrey D. ; Maitland, Kristen C. / Detection of bacterial infection with a fiber optic microendoscope. European Conference on Biomedical Optics, ECBO 2011. 2011.
@inproceedings{d95bdbd7ad044475874844d72c27d12f,
title = "Detection of bacterial infection with a fiber optic microendoscope",
abstract = "We present the use of fiber optic microendoscopy to image bacterial infection in the skin and lungs using an animal model. The contact probe microendoscope we have constructed has a 4 μm resolution, a 750 μm field of view, and a 1 mm outer diameter. Well resolved regions of bacterial infection were imaged for subcutaneous inocula of 106 to 101 CFU and intra-tracheal inocula of 108 to 106 CFU. Results reveal a linear relationship between average fluorescence and CFU, suggesting potential for using this device for quantitative analysis. Detection limits of 104 CFU for skin samples and 107 CFU for lung tissue were determined. In addition, bacteria were also qualitatively visible in lung tissue down to 106 CFU. Confocal imaging was used to confirm the presence of bacteria in tissue samples.",
author = "Nooman Mufti and Ying Kong and Cirillo, {Jeffrey D.} and Maitland, {Kristen C.}",
year = "2011",
language = "English (US)",
isbn = "9780819486837",
booktitle = "European Conference on Biomedical Optics, ECBO 2011",

}

TY - GEN

T1 - Detection of bacterial infection with a fiber optic microendoscope

AU - Mufti, Nooman

AU - Kong, Ying

AU - Cirillo, Jeffrey D.

AU - Maitland, Kristen C.

PY - 2011

Y1 - 2011

N2 - We present the use of fiber optic microendoscopy to image bacterial infection in the skin and lungs using an animal model. The contact probe microendoscope we have constructed has a 4 μm resolution, a 750 μm field of view, and a 1 mm outer diameter. Well resolved regions of bacterial infection were imaged for subcutaneous inocula of 106 to 101 CFU and intra-tracheal inocula of 108 to 106 CFU. Results reveal a linear relationship between average fluorescence and CFU, suggesting potential for using this device for quantitative analysis. Detection limits of 104 CFU for skin samples and 107 CFU for lung tissue were determined. In addition, bacteria were also qualitatively visible in lung tissue down to 106 CFU. Confocal imaging was used to confirm the presence of bacteria in tissue samples.

AB - We present the use of fiber optic microendoscopy to image bacterial infection in the skin and lungs using an animal model. The contact probe microendoscope we have constructed has a 4 μm resolution, a 750 μm field of view, and a 1 mm outer diameter. Well resolved regions of bacterial infection were imaged for subcutaneous inocula of 106 to 101 CFU and intra-tracheal inocula of 108 to 106 CFU. Results reveal a linear relationship between average fluorescence and CFU, suggesting potential for using this device for quantitative analysis. Detection limits of 104 CFU for skin samples and 107 CFU for lung tissue were determined. In addition, bacteria were also qualitatively visible in lung tissue down to 106 CFU. Confocal imaging was used to confirm the presence of bacteria in tissue samples.

UR - http://www.scopus.com/inward/record.url?scp=84893533798&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893533798&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9780819486837

BT - European Conference on Biomedical Optics, ECBO 2011

ER -