Detection of basal levels and induction of delta opioid receptor mRNA in murine splenocytes

Burt Sharp, Nahid Shahabi, David McKean, Ming D. Li, Kathy McAllen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Activation of delta opioid receptors (DOR) modulates calcium mobilization, interleukin-2 production, chemotaxis and proliferation of T- lymphocytes. Recent reports indicate that lymphocytes and mononuclear cells may express mRNA transcripts for DOR. The investigations reported herein show that low levels of DOR were consistently detected by RT-PCR amplification of RNA from freshly obtained Balb/c murine splenocytes, both weanling and adult. Culturing cells without stimulation increased DOR levels and concanavalin A apparently reduced this; DOR was preferentially expressed in a T-cell- enriched fraction. Thus, the expression of DOR mRNA by unactivated splenocytes is modulated by culture and con A in the T-cell fraction.

Original languageEnglish (US)
Pages (from-to)198-202
Number of pages5
JournalJournal of Neuroimmunology
Volume78
Issue number1-2
DOIs
StatePublished - Sep 1 1997

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delta Opioid Receptor
Messenger RNA
T-Lymphocytes
Chemotaxis
Concanavalin A
Interleukin-2
Lymphocytes
RNA
Calcium
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Detection of basal levels and induction of delta opioid receptor mRNA in murine splenocytes. / Sharp, Burt; Shahabi, Nahid; McKean, David; Li, Ming D.; McAllen, Kathy.

In: Journal of Neuroimmunology, Vol. 78, No. 1-2, 01.09.1997, p. 198-202.

Research output: Contribution to journalArticle

Sharp, Burt ; Shahabi, Nahid ; McKean, David ; Li, Ming D. ; McAllen, Kathy. / Detection of basal levels and induction of delta opioid receptor mRNA in murine splenocytes. In: Journal of Neuroimmunology. 1997 ; Vol. 78, No. 1-2. pp. 198-202.
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