Detection of circulating immune complexes with a Raji cell enzyme immunoassay

Neal S. Rote, Michael Caudle

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The Raji cell assay for circulating immune complexes (CIC) is frequently the method of choice when the detection of large, complement fixing complexes is desired. We have developed an enzyme-linked immunosorbent assay modification (Raji-ELISA) of the Raji cell technique which is easy to perform, uses commercially available reagents and is more convenient than the conventional Raji cell radioimmune assay (Raji-RIA). Fourteen samples were tested by both assays and a good correlation was observed (P=0.05). Sera from patients with suspected immune complex associated diseases were tested in the Raji-ELISA. Unsensitized Raji cells gave a value of 1.25 μg of immune complex associated IgG/ml. Using 2 standard deviations as a cutoff to determine positivity, 2 of 32 healthy controls (6.2%) had elevated levels of circulating complexes. In our study population, 9 of 23 cancer patients (39.1%), 10 of 13 patients with autoimmune diseases (76.9%), 3 of 17 patients with positive rheumatoid factor titers (17.6%), 1 of 23 pregnant patients (4.3%), 1 of 5 preeclamptic patients (20%) and 9 of 30 other patients with suspected immune complex associated diseases (30%) had elevated levels of CIC.

Original languageEnglish (US)
Pages (from-to)33-42
Number of pages10
JournalJournal of Immunological Methods
Volume56
Issue number1
DOIs
StatePublished - Jan 14 1983
Externally publishedYes

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Antigen-Antibody Complex
Immunoenzyme Techniques
Immune Complex Diseases
Enzyme-Linked Immunosorbent Assay
Rheumatoid Factor
Autoimmune Diseases
Immunoglobulin G
Serum
Population
Neoplasms

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Detection of circulating immune complexes with a Raji cell enzyme immunoassay. / Rote, Neal S.; Caudle, Michael.

In: Journal of Immunological Methods, Vol. 56, No. 1, 14.01.1983, p. 33-42.

Research output: Contribution to journalArticle

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abstract = "The Raji cell assay for circulating immune complexes (CIC) is frequently the method of choice when the detection of large, complement fixing complexes is desired. We have developed an enzyme-linked immunosorbent assay modification (Raji-ELISA) of the Raji cell technique which is easy to perform, uses commercially available reagents and is more convenient than the conventional Raji cell radioimmune assay (Raji-RIA). Fourteen samples were tested by both assays and a good correlation was observed (P=0.05). Sera from patients with suspected immune complex associated diseases were tested in the Raji-ELISA. Unsensitized Raji cells gave a value of 1.25 μg of immune complex associated IgG/ml. Using 2 standard deviations as a cutoff to determine positivity, 2 of 32 healthy controls (6.2{\%}) had elevated levels of circulating complexes. In our study population, 9 of 23 cancer patients (39.1{\%}), 10 of 13 patients with autoimmune diseases (76.9{\%}), 3 of 17 patients with positive rheumatoid factor titers (17.6{\%}), 1 of 23 pregnant patients (4.3{\%}), 1 of 5 preeclamptic patients (20{\%}) and 9 of 30 other patients with suspected immune complex associated diseases (30{\%}) had elevated levels of CIC.",
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