Detection of functional interferon alpha receptors in human neuroendocrine tumor cell lines using a new monoclonal antibody.

A. Rosolen, O. R. Colamonici, Lawrence Pfeffer, L. Whitesell, R. Nordan, L. M. Neckers

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

While first described as antiviral agents, interferons (IFNs) exhibit significant antiproliferative and antitumor effects as well. IFN alpha has been successfully used in clinical trials to treat several malignancies, including leukemias and certain solid tumors. While many cell types have been studied for IFN alpha receptor expression, very little is known about receptor expression on human neuroendocrine cells. Using a novel anti-IFN alpha receptor monoclonal antibody, we examined IFN alpha receptor expression in 10 human cell lines derived from tumors of neuroendocrine origin, including neuroblastoma, neuroepithelioma and small cell lung carcinoma. All cell lines studied displayed a similar pattern of IFN alpha receptor expression and 5 of 8 cell lines demonstrated reduced thymidine incorporation following IFN alpha treatment. Addition of exogenous IFN alpha caused a decrease in IFN alpha receptor expression, while differentiating agents, such as phorbol esters and retinoic acid, induced an increase in receptor number without altering receptor affinity.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalEuropean Cytokine Network
Volume3
Issue number2
StatePublished - Mar 1 1992
Externally publishedYes

Fingerprint

Interferon alpha-beta Receptor
Neuroendocrine Cells
Neuroendocrine Tumors
Tumor Cell Line
Tumors
Interferon-alpha
Monoclonal Antibodies
Cells
Cell Line
Small Cell Lung Carcinoma
Phorbol Esters
Tretinoin
Neuroblastoma
Thymidine
Interferons
Antiviral Agents
Neoplasms
Leukemia
Clinical Trials

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry

Cite this

Detection of functional interferon alpha receptors in human neuroendocrine tumor cell lines using a new monoclonal antibody. / Rosolen, A.; Colamonici, O. R.; Pfeffer, Lawrence; Whitesell, L.; Nordan, R.; Neckers, L. M.

In: European Cytokine Network, Vol. 3, No. 2, 01.03.1992, p. 81-88.

Research output: Contribution to journalArticle

Rosolen, A. ; Colamonici, O. R. ; Pfeffer, Lawrence ; Whitesell, L. ; Nordan, R. ; Neckers, L. M. / Detection of functional interferon alpha receptors in human neuroendocrine tumor cell lines using a new monoclonal antibody. In: European Cytokine Network. 1992 ; Vol. 3, No. 2. pp. 81-88.
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