Detection of Helicobacter pylori DNA in the oral cavity and gastroduodenal system of a Venezuelan population

Alejandra Berroteran, Marianella Perrone, María Correnti, María E. Cavazza, Claudio Tombazzi, Rosa Goncalvez, Vicente Lecuna

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Dental plaque has been suggested as a reservoir for Helicobacter pylori but the hypothesis that the oral microflora may be a permanent reservoir of H. pylori is still controversial. The aims of this study were to determine the presence of H. pylori DINA in the gastric antrum and dental plaque of a Venezuelan population by PCR and to investigate the relationship between this infection and the oral hygiene index. Thirty-two patients from the Hospital Universitario de Caracas, attending for routine gastroscopy, and 20 asymptomatic subjects (control group) were evaluated. The patients' gingiva and plaque were assessed by the gingival and plaque indices of Sillness and Löe. Supragingival plaque was analysed by a PCR for a specific internal urease gene. Gastric antrum biopsies were taken for histological examination and PCR. H. pylori was detected in antral samples from 24 (75%) of 32 patients, all of whom had chronic gastritis. H. pylori was also detected in dental plaque samples of 12 (37.5%) of the 32 patients. In 7 (58%) of these 12 patients, H. pylori was identified in the gastric biopsy. Seven patients with chronic gastritis carried H. pylori in dental plaque and antral samples. Of these patients, four also had dysplasia and one had metaplasia. Three subjects in the control group were positive by PCR. In the present study there was no correlation between H. pylori infection and dental hygiene, dental caries, periodontal disease or use of dentures. The oral cavity may be a reservoir for H. pylori infection and oral secretions may be an important means of transmission of this micro-organism. H. pylori in dental plaque may represent a risk factor for gastrointestinal re-infection and ulcer relapse after antibiotic therapy.

Original languageEnglish (US)
Pages (from-to)764-770
Number of pages7
JournalJournal of Medical Microbiology
Volume51
Issue number9
DOIs
StatePublished - Jan 1 2002

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Helicobacter pylori
Mouth
Dental Plaque
DNA
Population
Pyloric Antrum
Polymerase Chain Reaction
Helicobacter Infections
Gastritis
Oral Hygiene Index
Biopsy
Periodontal Index
Gastroscopy
Control Groups
Urease
Dentures
Oral Hygiene
Metaplasia
Dental Caries
Gingiva

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Detection of Helicobacter pylori DNA in the oral cavity and gastroduodenal system of a Venezuelan population. / Berroteran, Alejandra; Perrone, Marianella; Correnti, María; Cavazza, María E.; Tombazzi, Claudio; Goncalvez, Rosa; Lecuna, Vicente.

In: Journal of Medical Microbiology, Vol. 51, No. 9, 01.01.2002, p. 764-770.

Research output: Contribution to journalArticle

Berroteran, Alejandra ; Perrone, Marianella ; Correnti, María ; Cavazza, María E. ; Tombazzi, Claudio ; Goncalvez, Rosa ; Lecuna, Vicente. / Detection of Helicobacter pylori DNA in the oral cavity and gastroduodenal system of a Venezuelan population. In: Journal of Medical Microbiology. 2002 ; Vol. 51, No. 9. pp. 764-770.
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abstract = "Dental plaque has been suggested as a reservoir for Helicobacter pylori but the hypothesis that the oral microflora may be a permanent reservoir of H. pylori is still controversial. The aims of this study were to determine the presence of H. pylori DINA in the gastric antrum and dental plaque of a Venezuelan population by PCR and to investigate the relationship between this infection and the oral hygiene index. Thirty-two patients from the Hospital Universitario de Caracas, attending for routine gastroscopy, and 20 asymptomatic subjects (control group) were evaluated. The patients' gingiva and plaque were assessed by the gingival and plaque indices of Sillness and L{\"o}e. Supragingival plaque was analysed by a PCR for a specific internal urease gene. Gastric antrum biopsies were taken for histological examination and PCR. H. pylori was detected in antral samples from 24 (75{\%}) of 32 patients, all of whom had chronic gastritis. H. pylori was also detected in dental plaque samples of 12 (37.5{\%}) of the 32 patients. In 7 (58{\%}) of these 12 patients, H. pylori was identified in the gastric biopsy. Seven patients with chronic gastritis carried H. pylori in dental plaque and antral samples. Of these patients, four also had dysplasia and one had metaplasia. Three subjects in the control group were positive by PCR. In the present study there was no correlation between H. pylori infection and dental hygiene, dental caries, periodontal disease or use of dentures. The oral cavity may be a reservoir for H. pylori infection and oral secretions may be an important means of transmission of this micro-organism. H. pylori in dental plaque may represent a risk factor for gastrointestinal re-infection and ulcer relapse after antibiotic therapy.",
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