Detection of inflammation and oxidative lung injury in exhaled breath condensate of rats with acute lung injury due to Staphylococcal enterotoxin B

Rekha Solomon, Hitesh Sandhu, Suwannee Phumeetham, Keshava Murthy Narayana Gowda, Sabrina M. Heidemann

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The objectives were to determine if tumor necrosis factor (TNF)-α, leukotriene (LT)B4 and 8-isoprostane (IP) were present in the exhaled breath condensate (EBC) and total lung lavage (TLL) of mechanically ventilated rats, 4 and 24 h after administration of Staphylococcal entertoxin (SEB) and to find out if these mediators in the EBC correlate with the concentration in the TLL. Rats were assigned to control (n = 8); 4 h (n = 8) or 24 h (n = 8) groups after SEB. The rats were mechanically ventilated and EBC and TLL were collected for TNF-α, LTB4 and 8-IP. TNF-α was higher in the EBC of rats at 24 h after SEB when compared to control [34.5 (16-62 versus 2 (14-30) pg ml -1, p < 0.05] and also in the TLL [113.5 (70-460) versus 59 (35-79) pg ml-1, p < 0.04]. LTB4 was higher in the EBC of 24 h SEB rats, when compared to control [42 (28-50) versus 36 (29-37) pg ml -1, p < 0.01] and also in the TLL [179 (116-232) versus 114 (80-187) pg ml-1, p < 0.05). 8-IP was similar among the groups. No correlation was observed between TNF-α, LTB4 or 8-IP in the EBC compared to the TLL. TNF-α and LTB4 may be indicators of inflammation and oxidative lung injury but the EBC does not correlate with TLL concentrations.

Original languageEnglish (US)
Article number026003
JournalJournal of Breath Research
Volume7
Issue number2
DOIs
StatePublished - Jun 1 2013
Externally publishedYes

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8-epi-prostaglandin F2alpha
Acute Lung Injury
Lung Injury
Bronchoalveolar Lavage
Leukotriene B4
Inflammation
Tumor Necrosis Factor-alpha
staphylococcal enterotoxin B

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Detection of inflammation and oxidative lung injury in exhaled breath condensate of rats with acute lung injury due to Staphylococcal enterotoxin B. / Solomon, Rekha; Sandhu, Hitesh; Phumeetham, Suwannee; Gowda, Keshava Murthy Narayana; Heidemann, Sabrina M.

In: Journal of Breath Research, Vol. 7, No. 2, 026003, 01.06.2013.

Research output: Contribution to journalArticle

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