Detection of intrauterine viral infection using the polymerase chain reaction

Ignatia B. Van Den Veyver, Jiyuan Ni, Neil Bowles, Robert J. Carpenter, Carl P. Weiner, Jerome Yankowitz, Kenneth J. Moise, Janice Henderson, Jeffrey Towbin

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Intrauterine viral infection commonly presents as nonimmune hydrops fetalis or intrauterine growth restriction. Cytomegalovirus (CMV) and parvovirus are commonly recognized causes of fetal infection using serology and cultures. We used the polymerase chain reaction (PCR) to evaluate the frequency of fetal vital infection and the associated clinical course and outcome. Specimens (amniotic fluid, fetal blood, pleural fluid, tissue) from 303 abnormal pregnancies at risk for vital infection and 154 controls were analyzed using primers for CMV, herpes simplex virus, parvovirus B19, adenovirus, enterovirus, Epstein-Barr virus, and respiratory syncytial virus. Viral genome was detected in 144/371 samples (39%) or 124/303 patients (41%), with adenovirus (n = 74 patients; 24%), CMV (n = 30 patients; 10%), and enterovirus (n = 22 patients; 7%) most common. Only 4/154 (2.6%), unaffected control patients' samples were PCR positive. We conclude that diagnosis of fetal vital infection by PCR is common in abnormal pregnancies. Adenovirus and enterovirus may cause fetal infection that have been previously unrecognized.

Original languageEnglish (US)
Pages (from-to)85-95
Number of pages11
JournalMolecular Genetics and Metabolism
Volume63
Issue number2
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

Fingerprint

Polymerase chain reaction
Virus Diseases
Viruses
Enterovirus
Polymerase Chain Reaction
Cytomegalovirus
Adenoviridae
Parvovirus
Infection
Fluids
Blood
Hydrops Fetalis
Genes
Pregnancy
Respiratory Syncytial Viruses
Tissue
Viral Genome
Simplexvirus
Serology
Amniotic Fluid

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

Cite this

Van Den Veyver, I. B., Ni, J., Bowles, N., Carpenter, R. J., Weiner, C. P., Yankowitz, J., ... Towbin, J. (1998). Detection of intrauterine viral infection using the polymerase chain reaction. Molecular Genetics and Metabolism, 63(2), 85-95. https://doi.org/10.1006/mgme.1997.2651

Detection of intrauterine viral infection using the polymerase chain reaction. / Van Den Veyver, Ignatia B.; Ni, Jiyuan; Bowles, Neil; Carpenter, Robert J.; Weiner, Carl P.; Yankowitz, Jerome; Moise, Kenneth J.; Henderson, Janice; Towbin, Jeffrey.

In: Molecular Genetics and Metabolism, Vol. 63, No. 2, 01.01.1998, p. 85-95.

Research output: Contribution to journalArticle

Van Den Veyver, IB, Ni, J, Bowles, N, Carpenter, RJ, Weiner, CP, Yankowitz, J, Moise, KJ, Henderson, J & Towbin, J 1998, 'Detection of intrauterine viral infection using the polymerase chain reaction', Molecular Genetics and Metabolism, vol. 63, no. 2, pp. 85-95. https://doi.org/10.1006/mgme.1997.2651
Van Den Veyver IB, Ni J, Bowles N, Carpenter RJ, Weiner CP, Yankowitz J et al. Detection of intrauterine viral infection using the polymerase chain reaction. Molecular Genetics and Metabolism. 1998 Jan 1;63(2):85-95. https://doi.org/10.1006/mgme.1997.2651
Van Den Veyver, Ignatia B. ; Ni, Jiyuan ; Bowles, Neil ; Carpenter, Robert J. ; Weiner, Carl P. ; Yankowitz, Jerome ; Moise, Kenneth J. ; Henderson, Janice ; Towbin, Jeffrey. / Detection of intrauterine viral infection using the polymerase chain reaction. In: Molecular Genetics and Metabolism. 1998 ; Vol. 63, No. 2. pp. 85-95.
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