Detection of Lutetium Texaphyrin (Lutex) using its absorption of tissue autofluorescence

Masoud Panjehpour, Timothy Panella, Joseph DeCosta, William Moye, Markus Renschler

Research output: Contribution to journalConference article

Abstract

Laser induced fluorescence technique was used for detection of Lutetium Texaphyrin (Lutex) is vivo. Lutex is a new generation photosensitizer with rapid tissue clearance and deep red absorption characteristic. The detection system consisted of a nitrogen/dye laser (410 nm), an optical multichannel analyzer equipped with an intensified diode array, a specially designed fiber bundle, and a computer. Tissue fluorescence was first measured before injection of the Lutex to establish a baseline spectrum for each tissue. The fluorescence was then measured at 1, 2, 3, 4, 5, 6, 7, 8, 24, 48, 72 hours, 1, 2, 3, and 4 weeks after the injection. All spectra were normalized with respect to the total photon counts to determine the lineshape. The baseline was subtracted from all other fluorescence spectra. The Differential Spectrum showed a negative peak around 478 nm where the fluorescence of tissue was maximally absorbed by Lutex. Fluorescence was measured from mucus membrane of the lip, skin, and tumor in one case. Data for lip and tumor are presented here. As Lutex cleared the tissue, the negative peak at 478 nm approached zero. The clearance of the negative peak is presented for both lip (normal tissue) and nodular cutaneous metastatic breast is presented. In summary, absorption of tissue auto-fluorescence by Lutetium Texaphyrin may be used to detect the in vivo drug non-invasively. This technique may be used to determine the clearance of the Lutex in a variety of tissues.

Original languageEnglish (US)
Pages (from-to)48-55
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3247
DOIs
StatePublished - Dec 1 1998
Externally publishedYes
EventOptical methods for Tumor Treatment and Detections: Mechanisms and Techniques in Photodynamic Therapy VII - San Jose, CA, United States
Duration: Jan 24 1998Jan 25 1998

Fingerprint

Autofluorescence
Lutetium
lutetium
Fluorescence
Absorption
Tissue
Clearance
fluorescence
clearances
Baseline
Tumor
Injection
Photosensitizer
Laser-induced Fluorescence
Fiber Bundle
Tumors
Dyes
tumors
Diode
Skin

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Detection of Lutetium Texaphyrin (Lutex) using its absorption of tissue autofluorescence. / Panjehpour, Masoud; Panella, Timothy; DeCosta, Joseph; Moye, William; Renschler, Markus.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3247, 01.12.1998, p. 48-55.

Research output: Contribution to journalConference article

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AU - Renschler, Markus

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