Detection of miRNA in cultured cells or xenograft tissues of breast cancer

Martin Brown, Meiyun Fan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

MicroRNA (miRNA) analysis has evolved over the past two decades to become a highly specialized field with broad-reaching applications across a multitude of diseases and cellular processes. The choice of an applicable approach for miRNA quantification will depend on a variety of factors such as cost, time constraints, and throughput. Here, we describe the methods of total RNA isolation, AGO2-bound RNA isolation, miRNA polyadenylation, miRNA-cDNA synthesis, and quantitative real-time polymerase chain reaction for the detection of known miRNAs in cultured cells or xenograft tissues of breast cancer.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages81-88
Number of pages8
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1406
ISSN (Print)1064-3745

Fingerprint

MicroRNAs
Heterografts
Cultured Cells
Breast Neoplasms
RNA
Polyadenylation
Real-Time Polymerase Chain Reaction
Complementary DNA
Costs and Cost Analysis

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Brown, M., & Fan, M. (2016). Detection of miRNA in cultured cells or xenograft tissues of breast cancer. In Methods in Molecular Biology (pp. 81-88). (Methods in Molecular Biology; Vol. 1406). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3444-7_7

Detection of miRNA in cultured cells or xenograft tissues of breast cancer. / Brown, Martin; Fan, Meiyun.

Methods in Molecular Biology. Humana Press Inc., 2016. p. 81-88 (Methods in Molecular Biology; Vol. 1406).

Research output: Chapter in Book/Report/Conference proceedingChapter

Brown, M & Fan, M 2016, Detection of miRNA in cultured cells or xenograft tissues of breast cancer. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1406, Humana Press Inc., pp. 81-88. https://doi.org/10.1007/978-1-4939-3444-7_7
Brown M, Fan M. Detection of miRNA in cultured cells or xenograft tissues of breast cancer. In Methods in Molecular Biology. Humana Press Inc. 2016. p. 81-88. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3444-7_7
Brown, Martin ; Fan, Meiyun. / Detection of miRNA in cultured cells or xenograft tissues of breast cancer. Methods in Molecular Biology. Humana Press Inc., 2016. pp. 81-88 (Methods in Molecular Biology).
@inbook{78699711e9024726a3346ede7d74226b,
title = "Detection of miRNA in cultured cells or xenograft tissues of breast cancer",
abstract = "MicroRNA (miRNA) analysis has evolved over the past two decades to become a highly specialized field with broad-reaching applications across a multitude of diseases and cellular processes. The choice of an applicable approach for miRNA quantification will depend on a variety of factors such as cost, time constraints, and throughput. Here, we describe the methods of total RNA isolation, AGO2-bound RNA isolation, miRNA polyadenylation, miRNA-cDNA synthesis, and quantitative real-time polymerase chain reaction for the detection of known miRNAs in cultured cells or xenograft tissues of breast cancer.",
author = "Martin Brown and Meiyun Fan",
year = "2016",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-3444-7_7",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "81--88",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Detection of miRNA in cultured cells or xenograft tissues of breast cancer

AU - Brown, Martin

AU - Fan, Meiyun

PY - 2016/1/1

Y1 - 2016/1/1

N2 - MicroRNA (miRNA) analysis has evolved over the past two decades to become a highly specialized field with broad-reaching applications across a multitude of diseases and cellular processes. The choice of an applicable approach for miRNA quantification will depend on a variety of factors such as cost, time constraints, and throughput. Here, we describe the methods of total RNA isolation, AGO2-bound RNA isolation, miRNA polyadenylation, miRNA-cDNA synthesis, and quantitative real-time polymerase chain reaction for the detection of known miRNAs in cultured cells or xenograft tissues of breast cancer.

AB - MicroRNA (miRNA) analysis has evolved over the past two decades to become a highly specialized field with broad-reaching applications across a multitude of diseases and cellular processes. The choice of an applicable approach for miRNA quantification will depend on a variety of factors such as cost, time constraints, and throughput. Here, we describe the methods of total RNA isolation, AGO2-bound RNA isolation, miRNA polyadenylation, miRNA-cDNA synthesis, and quantitative real-time polymerase chain reaction for the detection of known miRNAs in cultured cells or xenograft tissues of breast cancer.

UR - http://www.scopus.com/inward/record.url?scp=84976240372&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84976240372&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3444-7_7

DO - 10.1007/978-1-4939-3444-7_7

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 81

EP - 88

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -