Detection of proliferating S-phase brain tumor cells by in situ DNA replication

R. J. Weil, S. A. Toms, Mahlon Johnson, A. Mealer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Object. Current methods used to describe the proliferative status of brain tumors rely on labor-intensive, potentially costly procedures. This article provides a description of a rapid, inexpensive, uncomplicated technique used to identify proliferating cells in tissue obtained at the time of resection. Methods. Touch preparations of 16 fresh astrocytic tumors and four fresh healthy temporal neocortical tissue samples were obtained at the time of surgery. Slides were placed in hypotonic potassium chloride to permeabilize their membranes, incubated in nucleotide precursors, and labeled with bromodeoxyuridine; they were later examined with the aid of a fluorescence microscope. The percentage of tumor cells in the S phase increased in conjunction with the grade of tumor and corresponded with the findings of immunohistochemical staining for the cell-cycle marker MIB-1. These results were confirmed in cell culture by using normal human astrocytes and two glioma cell lines. Slides can be analyzed in as little as 30 minutes after removal of tissue during surgery. Conclusions. In this study the authors describe a simple method by which cells in the S phase of the cell cycle, which are contained in fresh tumor obtained at the time of surgery, can be labeled. This method may prove a useful adjunct to frozen-section analysis and may permit discrimination of neoplastic tissues from other tissues observed in small specimen samples.

Original languageEnglish (US)
Pages (from-to)833-838
Number of pages6
JournalJournal of neurosurgery
Volume95
Issue number5
DOIs
StatePublished - Jan 1 2001

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DNA Replication
S Phase
Brain Neoplasms
Neoplasms
Cell Cycle
Potassium Chloride
Touch
Frozen Sections
Bromodeoxyuridine
Glioma
Astrocytes
Nucleotides
Cell Culture Techniques
Fluorescence
Staining and Labeling
Cell Line
Membranes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Clinical Neurology

Cite this

Detection of proliferating S-phase brain tumor cells by in situ DNA replication. / Weil, R. J.; Toms, S. A.; Johnson, Mahlon; Mealer, A.

In: Journal of neurosurgery, Vol. 95, No. 5, 01.01.2001, p. 833-838.

Research output: Contribution to journalArticle

Weil, R. J. ; Toms, S. A. ; Johnson, Mahlon ; Mealer, A. / Detection of proliferating S-phase brain tumor cells by in situ DNA replication. In: Journal of neurosurgery. 2001 ; Vol. 95, No. 5. pp. 833-838.
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