Detection of tumor necrosis factor-α and interleukin-6 in exhaled breath condensate of rats with pneumonia due to staphylococcal enterotoxin B

Sabrina M. Heidemann, Hitesh Sandhu, Natalija Kovacevic, Suwannee Phumeetham, Rekha Solomon

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Exhaled breath condensate (EBC) may contain mediators of acute lung injury. The objectives were to determine if EBC could be collected in a mechanically ventilated rat, to measure tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the EBC after staphylococcal enterotoxin B administration (SEB) and to find out if the concentrations of TNF-α and IL-6 correlated with those in lung lavage. Four hours after SEB instillation, rats were placed on mechanical ventilation and EBC was collected over 90 minutes. Lung lavage was collected and white cell count was determined. TNF-α and IL-6 were measured in the EBC and lavage. EBC was available in a sufficient quantity (250-400 μL) for the measurement of cytokines. The rats that received SEB had an inflammatory response when compared to control rats as shown by an increase in white cell count. TNF-α and IL-6 were detected in the EBC. Concentration of TNF-α correlated with that in the lavage (r = .497, P = .021), whereas IL-6 did not. EBC can be collected in rats in sufficient quantities to study acute lung injury. TNF-α and IL-6 can be measured in the EBC. Correlation between TNF-α in the EBC and lavage was demonstrated in this rat model of lung injury.

Original languageEnglish (US)
Pages (from-to)563-567
Number of pages5
JournalExperimental Lung Research
Volume37
Issue number9
DOIs
StatePublished - Nov 1 2011

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Rats
Interleukin-6
Pneumonia
Tumor Necrosis Factor-alpha
Therapeutic Irrigation
Acute Lung Injury
Bronchoalveolar Lavage
Cell Count
Rat control
Lung Injury
staphylococcal enterotoxin B
Artificial Respiration
Cytokines

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry

Cite this

Detection of tumor necrosis factor-α and interleukin-6 in exhaled breath condensate of rats with pneumonia due to staphylococcal enterotoxin B. / Heidemann, Sabrina M.; Sandhu, Hitesh; Kovacevic, Natalija; Phumeetham, Suwannee; Solomon, Rekha.

In: Experimental Lung Research, Vol. 37, No. 9, 01.11.2011, p. 563-567.

Research output: Contribution to journalArticle

Heidemann, Sabrina M. ; Sandhu, Hitesh ; Kovacevic, Natalija ; Phumeetham, Suwannee ; Solomon, Rekha. / Detection of tumor necrosis factor-α and interleukin-6 in exhaled breath condensate of rats with pneumonia due to staphylococcal enterotoxin B. In: Experimental Lung Research. 2011 ; Vol. 37, No. 9. pp. 563-567.
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