Determination of caries risk at resin composite margins

Norbert Krämer, Karl Heinz Kunzelmann, Franklin Garcia-Godoy, Ingo Häberlein, Bettina Meier, Roland Frankenberger

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Purpose: To design an artificial mouth in order to evaluate if a new diagnostic tool (Clinpro Cario Diagnosis) can be used for early detection of secondary caries at resin composite margins in vitro. Methods: 32 intact human third molars received standardized Class-V resin composite restorations (Tetric Ceram bonded with Syntac SC). After storage for 4 weeks at 37°C, teeth were subjected to 5,000 or 10,000 thermocycles (±5°C and ±55°C) and polysiloxane impressions were taken. Streptococcus mutans 10449 (SM) was used in a nutrition medium to initiate a secondary caries process. Daily, the teeth were incubated for 2 x 2.5 hours in SM containing nutrition medium followed by 2 x 9.5 hours incubation in artificial saliva. Teeth were investigated after total incubation periods of 4, 6, and 8 weeks. After the different incubation protocols, the restoration margins were evaluated for infection and secondary caries processes in using Clinpro Cario Diagnosis which measures site-specifically the lactic acid production of SM in response to a sucrose challenge. The color signal was read 5 minutes after removal of the diagnostic impression. After thermocycling and biological load cycling, precision polysiloxane impressions were taken and replicas were investigated under a light microscope for gap widths at enamel and dentin margins. Demineralization was evaluated by fluorescence microscopy in using a special FITC filter. The demineralization depths at the cavity margin were calculated with Xpert for Windows using a pixel distance of 5 μm. Results: After the different thermocycling protocols, no differences in gap widths and demineralization depths were found (P> 0.05). After SM incubation, gap widths and demineralization depths were significantly dependent on SM incubation time and previous number of thermocycles (P< 0.05). Lactic acid formations of SM were detectable by Clinpro Cario Diagnosis at dentin cavosurface margins formed after 6 weeks of incubation with SM (P< 0.05).

Original languageEnglish (US)
Pages (from-to)59-64
Number of pages6
JournalAmerican Journal of Dentistry
Volume20
Issue number1
StatePublished - Feb 1 2007
Externally publishedYes

Fingerprint

Streptococcus mutans
Composite Resins
Siloxanes
Tooth
Dentin
Lactic Acid
Artificial Saliva
Third Molar
Fluorescein-5-isothiocyanate
Dental Enamel
Coinfection
Fluorescence Microscopy
Sucrose
Mouth
Color
Light

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Krämer, N., Kunzelmann, K. H., Garcia-Godoy, F., Häberlein, I., Meier, B., & Frankenberger, R. (2007). Determination of caries risk at resin composite margins. American Journal of Dentistry, 20(1), 59-64.

Determination of caries risk at resin composite margins. / Krämer, Norbert; Kunzelmann, Karl Heinz; Garcia-Godoy, Franklin; Häberlein, Ingo; Meier, Bettina; Frankenberger, Roland.

In: American Journal of Dentistry, Vol. 20, No. 1, 01.02.2007, p. 59-64.

Research output: Contribution to journalArticle

Krämer, N, Kunzelmann, KH, Garcia-Godoy, F, Häberlein, I, Meier, B & Frankenberger, R 2007, 'Determination of caries risk at resin composite margins', American Journal of Dentistry, vol. 20, no. 1, pp. 59-64.
Krämer N, Kunzelmann KH, Garcia-Godoy F, Häberlein I, Meier B, Frankenberger R. Determination of caries risk at resin composite margins. American Journal of Dentistry. 2007 Feb 1;20(1):59-64.
Krämer, Norbert ; Kunzelmann, Karl Heinz ; Garcia-Godoy, Franklin ; Häberlein, Ingo ; Meier, Bettina ; Frankenberger, Roland. / Determination of caries risk at resin composite margins. In: American Journal of Dentistry. 2007 ; Vol. 20, No. 1. pp. 59-64.
@article{fc96a1fe75384352ab2a60f37aebe947,
title = "Determination of caries risk at resin composite margins",
abstract = "Purpose: To design an artificial mouth in order to evaluate if a new diagnostic tool (Clinpro Cario Diagnosis) can be used for early detection of secondary caries at resin composite margins in vitro. Methods: 32 intact human third molars received standardized Class-V resin composite restorations (Tetric Ceram bonded with Syntac SC). After storage for 4 weeks at 37°C, teeth were subjected to 5,000 or 10,000 thermocycles (±5°C and ±55°C) and polysiloxane impressions were taken. Streptococcus mutans 10449 (SM) was used in a nutrition medium to initiate a secondary caries process. Daily, the teeth were incubated for 2 x 2.5 hours in SM containing nutrition medium followed by 2 x 9.5 hours incubation in artificial saliva. Teeth were investigated after total incubation periods of 4, 6, and 8 weeks. After the different incubation protocols, the restoration margins were evaluated for infection and secondary caries processes in using Clinpro Cario Diagnosis which measures site-specifically the lactic acid production of SM in response to a sucrose challenge. The color signal was read 5 minutes after removal of the diagnostic impression. After thermocycling and biological load cycling, precision polysiloxane impressions were taken and replicas were investigated under a light microscope for gap widths at enamel and dentin margins. Demineralization was evaluated by fluorescence microscopy in using a special FITC filter. The demineralization depths at the cavity margin were calculated with Xpert for Windows using a pixel distance of 5 μm. Results: After the different thermocycling protocols, no differences in gap widths and demineralization depths were found (P> 0.05). After SM incubation, gap widths and demineralization depths were significantly dependent on SM incubation time and previous number of thermocycles (P< 0.05). Lactic acid formations of SM were detectable by Clinpro Cario Diagnosis at dentin cavosurface margins formed after 6 weeks of incubation with SM (P< 0.05).",
author = "Norbert Kr{\"a}mer and Kunzelmann, {Karl Heinz} and Franklin Garcia-Godoy and Ingo H{\"a}berlein and Bettina Meier and Roland Frankenberger",
year = "2007",
month = "2",
day = "1",
language = "English (US)",
volume = "20",
pages = "59--64",
journal = "American Journal of Dentistry",
issn = "0894-8275",
publisher = "Mosher and Linder, Inc",
number = "1",

}

TY - JOUR

T1 - Determination of caries risk at resin composite margins

AU - Krämer, Norbert

AU - Kunzelmann, Karl Heinz

AU - Garcia-Godoy, Franklin

AU - Häberlein, Ingo

AU - Meier, Bettina

AU - Frankenberger, Roland

PY - 2007/2/1

Y1 - 2007/2/1

N2 - Purpose: To design an artificial mouth in order to evaluate if a new diagnostic tool (Clinpro Cario Diagnosis) can be used for early detection of secondary caries at resin composite margins in vitro. Methods: 32 intact human third molars received standardized Class-V resin composite restorations (Tetric Ceram bonded with Syntac SC). After storage for 4 weeks at 37°C, teeth were subjected to 5,000 or 10,000 thermocycles (±5°C and ±55°C) and polysiloxane impressions were taken. Streptococcus mutans 10449 (SM) was used in a nutrition medium to initiate a secondary caries process. Daily, the teeth were incubated for 2 x 2.5 hours in SM containing nutrition medium followed by 2 x 9.5 hours incubation in artificial saliva. Teeth were investigated after total incubation periods of 4, 6, and 8 weeks. After the different incubation protocols, the restoration margins were evaluated for infection and secondary caries processes in using Clinpro Cario Diagnosis which measures site-specifically the lactic acid production of SM in response to a sucrose challenge. The color signal was read 5 minutes after removal of the diagnostic impression. After thermocycling and biological load cycling, precision polysiloxane impressions were taken and replicas were investigated under a light microscope for gap widths at enamel and dentin margins. Demineralization was evaluated by fluorescence microscopy in using a special FITC filter. The demineralization depths at the cavity margin were calculated with Xpert for Windows using a pixel distance of 5 μm. Results: After the different thermocycling protocols, no differences in gap widths and demineralization depths were found (P> 0.05). After SM incubation, gap widths and demineralization depths were significantly dependent on SM incubation time and previous number of thermocycles (P< 0.05). Lactic acid formations of SM were detectable by Clinpro Cario Diagnosis at dentin cavosurface margins formed after 6 weeks of incubation with SM (P< 0.05).

AB - Purpose: To design an artificial mouth in order to evaluate if a new diagnostic tool (Clinpro Cario Diagnosis) can be used for early detection of secondary caries at resin composite margins in vitro. Methods: 32 intact human third molars received standardized Class-V resin composite restorations (Tetric Ceram bonded with Syntac SC). After storage for 4 weeks at 37°C, teeth were subjected to 5,000 or 10,000 thermocycles (±5°C and ±55°C) and polysiloxane impressions were taken. Streptococcus mutans 10449 (SM) was used in a nutrition medium to initiate a secondary caries process. Daily, the teeth were incubated for 2 x 2.5 hours in SM containing nutrition medium followed by 2 x 9.5 hours incubation in artificial saliva. Teeth were investigated after total incubation periods of 4, 6, and 8 weeks. After the different incubation protocols, the restoration margins were evaluated for infection and secondary caries processes in using Clinpro Cario Diagnosis which measures site-specifically the lactic acid production of SM in response to a sucrose challenge. The color signal was read 5 minutes after removal of the diagnostic impression. After thermocycling and biological load cycling, precision polysiloxane impressions were taken and replicas were investigated under a light microscope for gap widths at enamel and dentin margins. Demineralization was evaluated by fluorescence microscopy in using a special FITC filter. The demineralization depths at the cavity margin were calculated with Xpert for Windows using a pixel distance of 5 μm. Results: After the different thermocycling protocols, no differences in gap widths and demineralization depths were found (P> 0.05). After SM incubation, gap widths and demineralization depths were significantly dependent on SM incubation time and previous number of thermocycles (P< 0.05). Lactic acid formations of SM were detectable by Clinpro Cario Diagnosis at dentin cavosurface margins formed after 6 weeks of incubation with SM (P< 0.05).

UR - http://www.scopus.com/inward/record.url?scp=33947361530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947361530&partnerID=8YFLogxK

M3 - Article

VL - 20

SP - 59

EP - 64

JO - American Journal of Dentistry

JF - American Journal of Dentistry

SN - 0894-8275

IS - 1

ER -