Determination of quercetin metabolites in rat plasma by liquid chromatography-mass spectrometry

Feng Chen, Nai Guang Fu, Shou Zhong Ren, Na Wei, Jun Qing Zhang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

OBJECTIVE: To determine the metabolites of quercetin in rat plasma after an oral administration by Liquid Chromatography-Mass Spectrometry(LC-MS). METHODS: The rats were administrated with quercetin monomer solution(100 mg·kg-1)oral and the blood sample were collected. Quercetin and isorhamnetin were extracted from rat plasma after acid hydrolysis using 2 mol·L-1 HCl in aqueous methonl. After acid hydrolysis, the analytes of interest were separated on a SUPELCOSIL™ C18 column. The mobile phase was methonl-acetonitrile-1% acetic acid(20:30:50) with a flow rate of 0.8 mL·min-1. A Bruker Mass Spectrometry equipped with an Atmospheric Pressure Ionization-Electrospray (EPI) source was used as detector and was operated in negative ion mode. RESULTS: Quercetin and isorhamnetin were identified using the standard spectrum and the results of mass spectrometry, i. e. m/z→301.0 for quercetin and m/z→314.9 for isorhamnetin. CONCLUSION: Acetic acid hydrolysis was a fast feasible method and could be used to identify the quercetin metabolites in rat plasma. And this method could be a guidance for pharmacokinetics studie upon Traditional Chinese Medicines.

Original languageEnglish (US)
JournalChinese Pharmaceutical Journal
Volume43
Issue number3
StatePublished - Feb 1 2008

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Quercetin
Liquid Chromatography
Mass Spectrometry
Hydrolysis
Acetic Acid
Atmospheric Pressure
Acids
Chinese Traditional Medicine
Oral Administration
Pharmacokinetics
Ions
3-methylquercetin

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Determination of quercetin metabolites in rat plasma by liquid chromatography-mass spectrometry. / Chen, Feng; Fu, Nai Guang; Ren, Shou Zhong; Wei, Na; Zhang, Jun Qing.

In: Chinese Pharmaceutical Journal, Vol. 43, No. 3, 01.02.2008.

Research output: Contribution to journalArticle

Chen, Feng ; Fu, Nai Guang ; Ren, Shou Zhong ; Wei, Na ; Zhang, Jun Qing. / Determination of quercetin metabolites in rat plasma by liquid chromatography-mass spectrometry. In: Chinese Pharmaceutical Journal. 2008 ; Vol. 43, No. 3.
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abstract = "OBJECTIVE: To determine the metabolites of quercetin in rat plasma after an oral administration by Liquid Chromatography-Mass Spectrometry(LC-MS). METHODS: The rats were administrated with quercetin monomer solution(100 mg·kg-1)oral and the blood sample were collected. Quercetin and isorhamnetin were extracted from rat plasma after acid hydrolysis using 2 mol·L-1 HCl in aqueous methonl. After acid hydrolysis, the analytes of interest were separated on a SUPELCOSIL™ C18 column. The mobile phase was methonl-acetonitrile-1{\%} acetic acid(20:30:50) with a flow rate of 0.8 mL·min-1. A Bruker Mass Spectrometry equipped with an Atmospheric Pressure Ionization-Electrospray (EPI) source was used as detector and was operated in negative ion mode. RESULTS: Quercetin and isorhamnetin were identified using the standard spectrum and the results of mass spectrometry, i. e. m/z→301.0 for quercetin and m/z→314.9 for isorhamnetin. CONCLUSION: Acetic acid hydrolysis was a fast feasible method and could be used to identify the quercetin metabolites in rat plasma. And this method could be a guidance for pharmacokinetics studie upon Traditional Chinese Medicines.",
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N2 - OBJECTIVE: To determine the metabolites of quercetin in rat plasma after an oral administration by Liquid Chromatography-Mass Spectrometry(LC-MS). METHODS: The rats were administrated with quercetin monomer solution(100 mg·kg-1)oral and the blood sample were collected. Quercetin and isorhamnetin were extracted from rat plasma after acid hydrolysis using 2 mol·L-1 HCl in aqueous methonl. After acid hydrolysis, the analytes of interest were separated on a SUPELCOSIL™ C18 column. The mobile phase was methonl-acetonitrile-1% acetic acid(20:30:50) with a flow rate of 0.8 mL·min-1. A Bruker Mass Spectrometry equipped with an Atmospheric Pressure Ionization-Electrospray (EPI) source was used as detector and was operated in negative ion mode. RESULTS: Quercetin and isorhamnetin were identified using the standard spectrum and the results of mass spectrometry, i. e. m/z→301.0 for quercetin and m/z→314.9 for isorhamnetin. CONCLUSION: Acetic acid hydrolysis was a fast feasible method and could be used to identify the quercetin metabolites in rat plasma. And this method could be a guidance for pharmacokinetics studie upon Traditional Chinese Medicines.

AB - OBJECTIVE: To determine the metabolites of quercetin in rat plasma after an oral administration by Liquid Chromatography-Mass Spectrometry(LC-MS). METHODS: The rats were administrated with quercetin monomer solution(100 mg·kg-1)oral and the blood sample were collected. Quercetin and isorhamnetin were extracted from rat plasma after acid hydrolysis using 2 mol·L-1 HCl in aqueous methonl. After acid hydrolysis, the analytes of interest were separated on a SUPELCOSIL™ C18 column. The mobile phase was methonl-acetonitrile-1% acetic acid(20:30:50) with a flow rate of 0.8 mL·min-1. A Bruker Mass Spectrometry equipped with an Atmospheric Pressure Ionization-Electrospray (EPI) source was used as detector and was operated in negative ion mode. RESULTS: Quercetin and isorhamnetin were identified using the standard spectrum and the results of mass spectrometry, i. e. m/z→301.0 for quercetin and m/z→314.9 for isorhamnetin. CONCLUSION: Acetic acid hydrolysis was a fast feasible method and could be used to identify the quercetin metabolites in rat plasma. And this method could be a guidance for pharmacokinetics studie upon Traditional Chinese Medicines.

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