Development and in vitro studies of a polyethylene terephthalate-gold nanoparticle scaffold for improved biocompatibility

Ona E. Whelove, Matthew J. Cozad, Byung Doo Lee, Shramik Sengupta, Sharon L. Bachman, Bruce Ramshaw, Sheila A. Grant

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Polyethylene terephthalate (PET) mesh is one of the most commonly used synthetic biomaterials for tension-free hernia repair. In an effort to improve the biocompatibility of PET mesh, gold nanoparticles (AuNP) in various concentrations were conjugated to the PET surface to develop PET-AuNP scaffolds. These novel scaffolds were characterized with Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC) to assess the addition of functional groups, presence of AuNPs, and thermal stability of the modified PET mesh, respectively. The biocompatibility of the PET-AuNP scaffolds was evaluated through in vitro cell culture assays. The cellularity of cells exposed to the PET-AuNP scaffolds, as well as the scaffolds' ability to reduce reactive oxygen species, was assessed using L929 murine fibroblasts. Antimicrobial properties of AuNPs conjugated to PET mesh were tested against the bacteria Pseudomonas aeruginosa. Results from the FT-IR showed presence of COOH groups while SEM displayed bonding of AuNPs to the PET surface. DSC results indicated that the PET more than likely did not undergo any detrimental degradation due to the surface modification. Results from the in vitro studies showed that AuNPs, in optimal concentrations (1× concentrations), enhanced cellularity, reduced ROS, and reduced bacteria adhesion to PET. These studies demonstrated enhanced biocompatibility of the AuNP conjugated PET mesh over pristine PET mesh.

Original languageEnglish (US)
Pages (from-to)142-149
Number of pages8
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume99 B
Issue number1
DOIs
StatePublished - Oct 2011
Externally publishedYes

Fingerprint

Polyethylene Terephthalates
Scaffolds (biology)
Biocompatibility
Gold
Polyethylene terephthalates
Nanoparticles
Scaffolds
Fourier transform infrared spectroscopy
Differential scanning calorimetry
Bacteria
Scanning electron microscopy
Biocompatible Materials
Fibroblasts
Cell culture
Biomaterials
Functional groups
Surface treatment
Assays
Reactive Oxygen Species
Thermodynamic stability

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Biomaterials

Cite this

Development and in vitro studies of a polyethylene terephthalate-gold nanoparticle scaffold for improved biocompatibility. / Whelove, Ona E.; Cozad, Matthew J.; Lee, Byung Doo; Sengupta, Shramik; Bachman, Sharon L.; Ramshaw, Bruce; Grant, Sheila A.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 99 B, No. 1, 10.2011, p. 142-149.

Research output: Contribution to journalArticle

Whelove, Ona E. ; Cozad, Matthew J. ; Lee, Byung Doo ; Sengupta, Shramik ; Bachman, Sharon L. ; Ramshaw, Bruce ; Grant, Sheila A. / Development and in vitro studies of a polyethylene terephthalate-gold nanoparticle scaffold for improved biocompatibility. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2011 ; Vol. 99 B, No. 1. pp. 142-149.
@article{c459126d3df1460e9aa1df103a5d77ef,
title = "Development and in vitro studies of a polyethylene terephthalate-gold nanoparticle scaffold for improved biocompatibility",
abstract = "Polyethylene terephthalate (PET) mesh is one of the most commonly used synthetic biomaterials for tension-free hernia repair. In an effort to improve the biocompatibility of PET mesh, gold nanoparticles (AuNP) in various concentrations were conjugated to the PET surface to develop PET-AuNP scaffolds. These novel scaffolds were characterized with Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC) to assess the addition of functional groups, presence of AuNPs, and thermal stability of the modified PET mesh, respectively. The biocompatibility of the PET-AuNP scaffolds was evaluated through in vitro cell culture assays. The cellularity of cells exposed to the PET-AuNP scaffolds, as well as the scaffolds' ability to reduce reactive oxygen species, was assessed using L929 murine fibroblasts. Antimicrobial properties of AuNPs conjugated to PET mesh were tested against the bacteria Pseudomonas aeruginosa. Results from the FT-IR showed presence of COOH groups while SEM displayed bonding of AuNPs to the PET surface. DSC results indicated that the PET more than likely did not undergo any detrimental degradation due to the surface modification. Results from the in vitro studies showed that AuNPs, in optimal concentrations (1× concentrations), enhanced cellularity, reduced ROS, and reduced bacteria adhesion to PET. These studies demonstrated enhanced biocompatibility of the AuNP conjugated PET mesh over pristine PET mesh.",
author = "Whelove, {Ona E.} and Cozad, {Matthew J.} and Lee, {Byung Doo} and Shramik Sengupta and Bachman, {Sharon L.} and Bruce Ramshaw and Grant, {Sheila A.}",
year = "2011",
month = "10",
doi = "10.1002/jbm.b.31881",
language = "English (US)",
volume = "99 B",
pages = "142--149",
journal = "Journal of Biomedical Materials Research",
issn = "1552-4973",
publisher = "Heterocorporation",
number = "1",

}

TY - JOUR

T1 - Development and in vitro studies of a polyethylene terephthalate-gold nanoparticle scaffold for improved biocompatibility

AU - Whelove, Ona E.

AU - Cozad, Matthew J.

AU - Lee, Byung Doo

AU - Sengupta, Shramik

AU - Bachman, Sharon L.

AU - Ramshaw, Bruce

AU - Grant, Sheila A.

PY - 2011/10

Y1 - 2011/10

N2 - Polyethylene terephthalate (PET) mesh is one of the most commonly used synthetic biomaterials for tension-free hernia repair. In an effort to improve the biocompatibility of PET mesh, gold nanoparticles (AuNP) in various concentrations were conjugated to the PET surface to develop PET-AuNP scaffolds. These novel scaffolds were characterized with Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC) to assess the addition of functional groups, presence of AuNPs, and thermal stability of the modified PET mesh, respectively. The biocompatibility of the PET-AuNP scaffolds was evaluated through in vitro cell culture assays. The cellularity of cells exposed to the PET-AuNP scaffolds, as well as the scaffolds' ability to reduce reactive oxygen species, was assessed using L929 murine fibroblasts. Antimicrobial properties of AuNPs conjugated to PET mesh were tested against the bacteria Pseudomonas aeruginosa. Results from the FT-IR showed presence of COOH groups while SEM displayed bonding of AuNPs to the PET surface. DSC results indicated that the PET more than likely did not undergo any detrimental degradation due to the surface modification. Results from the in vitro studies showed that AuNPs, in optimal concentrations (1× concentrations), enhanced cellularity, reduced ROS, and reduced bacteria adhesion to PET. These studies demonstrated enhanced biocompatibility of the AuNP conjugated PET mesh over pristine PET mesh.

AB - Polyethylene terephthalate (PET) mesh is one of the most commonly used synthetic biomaterials for tension-free hernia repair. In an effort to improve the biocompatibility of PET mesh, gold nanoparticles (AuNP) in various concentrations were conjugated to the PET surface to develop PET-AuNP scaffolds. These novel scaffolds were characterized with Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC) to assess the addition of functional groups, presence of AuNPs, and thermal stability of the modified PET mesh, respectively. The biocompatibility of the PET-AuNP scaffolds was evaluated through in vitro cell culture assays. The cellularity of cells exposed to the PET-AuNP scaffolds, as well as the scaffolds' ability to reduce reactive oxygen species, was assessed using L929 murine fibroblasts. Antimicrobial properties of AuNPs conjugated to PET mesh were tested against the bacteria Pseudomonas aeruginosa. Results from the FT-IR showed presence of COOH groups while SEM displayed bonding of AuNPs to the PET surface. DSC results indicated that the PET more than likely did not undergo any detrimental degradation due to the surface modification. Results from the in vitro studies showed that AuNPs, in optimal concentrations (1× concentrations), enhanced cellularity, reduced ROS, and reduced bacteria adhesion to PET. These studies demonstrated enhanced biocompatibility of the AuNP conjugated PET mesh over pristine PET mesh.

UR - http://www.scopus.com/inward/record.url?scp=80052307656&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052307656&partnerID=8YFLogxK

U2 - 10.1002/jbm.b.31881

DO - 10.1002/jbm.b.31881

M3 - Article

VL - 99 B

SP - 142

EP - 149

JO - Journal of Biomedical Materials Research

JF - Journal of Biomedical Materials Research

SN - 1552-4973

IS - 1

ER -