Development of functional B cells in a line of SCID mice with transgenes coding for anti-double-stranded DNA antibody

Gayle C. Bosma, Jennifer Oshinsky, Kerstin Kiefer, Pamela B. Nakajima, Deepshika Charan, Cecil Congelton, Marko Radic, Melvin J. Bosma

Research output: Contribution to journalArticle

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Abstract

Deletion or inactivation of anti-self (DNA) B cells has been reported in non-autoimmune mice bearing Ig transgenes that code for Abs with specificity for dsDNA or ssDNA. However, we report a case in which anti-dsDNA B cells appear to escape both deletion and inactivation. We show that B cells (B220 +IgM+) can develop in non-autoimmune SCID mice bearing two site-directed transgenes, 3H9(56R) and Vκ8, that together code for an anti-dsDNA Ab. The B cells appear inactive, because the mice (56RVκ8 SCID mice) generally lack serum Ig. However, 56RV κ8 SCID mice are able to produce IgG Ab with specificity for dsDNA when they become "leaky" for T cells or are reconstituted with exogenous T cells from B cell-deficient JH-/- donors. Thus, anti-dsDNA B cells that escape deletion in 56RV κ8 SCID mice appear fully functional and can differentiate, class switch, and give rise to IgG-producing cells in the presence of T cells and self-Ag.

Original languageEnglish (US)
Pages (from-to)889-898
Number of pages10
JournalJournal of Immunology
Volume176
Issue number2
DOIs
StatePublished - Jan 15 2006

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SCID Mice
Transgenes
B-Lymphocytes
Antibodies
DNA
T-Lymphocytes
Immunoglobulin G
Immunoglobulin M
Serum

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Bosma, G. C., Oshinsky, J., Kiefer, K., Nakajima, P. B., Charan, D., Congelton, C., ... Bosma, M. J. (2006). Development of functional B cells in a line of SCID mice with transgenes coding for anti-double-stranded DNA antibody. Journal of Immunology, 176(2), 889-898. https://doi.org/10.4049/jimmunol.176.2.889

Development of functional B cells in a line of SCID mice with transgenes coding for anti-double-stranded DNA antibody. / Bosma, Gayle C.; Oshinsky, Jennifer; Kiefer, Kerstin; Nakajima, Pamela B.; Charan, Deepshika; Congelton, Cecil; Radic, Marko; Bosma, Melvin J.

In: Journal of Immunology, Vol. 176, No. 2, 15.01.2006, p. 889-898.

Research output: Contribution to journalArticle

Bosma, GC, Oshinsky, J, Kiefer, K, Nakajima, PB, Charan, D, Congelton, C, Radic, M & Bosma, MJ 2006, 'Development of functional B cells in a line of SCID mice with transgenes coding for anti-double-stranded DNA antibody', Journal of Immunology, vol. 176, no. 2, pp. 889-898. https://doi.org/10.4049/jimmunol.176.2.889
Bosma, Gayle C. ; Oshinsky, Jennifer ; Kiefer, Kerstin ; Nakajima, Pamela B. ; Charan, Deepshika ; Congelton, Cecil ; Radic, Marko ; Bosma, Melvin J. / Development of functional B cells in a line of SCID mice with transgenes coding for anti-double-stranded DNA antibody. In: Journal of Immunology. 2006 ; Vol. 176, No. 2. pp. 889-898.
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