Developmental regulation and tissue-specific expression of a chimaeric phosphoenolpyruvate carboxykinase/bovine growth hormone gene in transgenic animals.

M. M. McGrane, J. S. Yun, W. J. Roesler, Edwards Park, T. E. Wagner, R. W. Hanson

Research output: Contribution to journalReview article

15 Citations (Scopus)

Abstract

Expression of the bovine growth hormone (bGH) gene, directed by the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter, in transgenic animals was investigated. Different lengths of the 5' PEPCK promoter-regulatory domain were utilized to control bGH expression; these included -2000/+73, -460/+73, -355/+73, and -174/+73. The chimaeric PEPCK/bGH gene containing -460/+73 of PEPCK 5' flanking sequence (PEPCK/bGH(460] is regulated by cAMP, insulin, and dexamethasone in the same manner as the endogenous PEPCK gene. This PEPCK promoter-regulatory domain also controls the tissue-specific expression of the bGH gene to liver, kidney, adipose tissue, jejunum and mammary gland. Furthermore, the correct developmental pattern of expression is observed in the mouse lines which contain PEPCK/bGH(460). The transgene mRNA is not detected during fetal development until Day 19. At Day 1 after birth, due to alterations in the insulin:glucagon ratio, the amounts of transgene mRNA are greatly increased, similar to the endogenous PEPCK gene.

Original languageEnglish (US)
Pages (from-to)17-23
Number of pages7
JournalJournal of reproduction and fertility. Supplement
Volume41
StatePublished - Jan 1 1990
Externally publishedYes

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Phosphoenolpyruvate
Genetically Modified Animals
Genes
Transgenes
Insulin
Messenger RNA
bovine growth hormone
5' Flanking Region
Jejunum
Human Mammary Glands
Fetal Development
Glucagon
Dexamethasone
Adipose Tissue
Parturition
Kidney
Liver

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Developmental regulation and tissue-specific expression of a chimaeric phosphoenolpyruvate carboxykinase/bovine growth hormone gene in transgenic animals. / McGrane, M. M.; Yun, J. S.; Roesler, W. J.; Park, Edwards; Wagner, T. E.; Hanson, R. W.

In: Journal of reproduction and fertility. Supplement, Vol. 41, 01.01.1990, p. 17-23.

Research output: Contribution to journalReview article

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abstract = "Expression of the bovine growth hormone (bGH) gene, directed by the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter, in transgenic animals was investigated. Different lengths of the 5' PEPCK promoter-regulatory domain were utilized to control bGH expression; these included -2000/+73, -460/+73, -355/+73, and -174/+73. The chimaeric PEPCK/bGH gene containing -460/+73 of PEPCK 5' flanking sequence (PEPCK/bGH(460] is regulated by cAMP, insulin, and dexamethasone in the same manner as the endogenous PEPCK gene. This PEPCK promoter-regulatory domain also controls the tissue-specific expression of the bGH gene to liver, kidney, adipose tissue, jejunum and mammary gland. Furthermore, the correct developmental pattern of expression is observed in the mouse lines which contain PEPCK/bGH(460). The transgene mRNA is not detected during fetal development until Day 19. At Day 1 after birth, due to alterations in the insulin:glucagon ratio, the amounts of transgene mRNA are greatly increased, similar to the endogenous PEPCK gene.",
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AU - Yun, J. S.

AU - Roesler, W. J.

AU - Park, Edwards

AU - Wagner, T. E.

AU - Hanson, R. W.

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