Developmental regulation and tissue-specific expression of hamster dolichol-P-dependent N-acetylglucosamine-1-P transferase (GPT)

Olivia M. Mota, George Huang, Maria A. Kukuruzinska

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The first enzyme in the dolichol pathway of protein N-glycosylation, dolichol-P-dependent N-acetylglucosamine-1-phosphate transferase, GPT, has been implicated in the development of a wide variety of eukaryotes. GPT is encoded by ALG7 an early growth-response gene, whose expression has been shown to affect the extent of N-glycosylation and secretion of proteins. To initiate the molecular characterization of ALG7 involvement in mammalian growth and differentiation, we have used the postnatally developing hamster submandibular gland (SMG) as an experimental paradigm. In this study we report that the ALG7 gene was differentially expressed during postnatal development and in terminally differentiated adult tissues. Throughout development, GPT activity paralleled ALG7 mRNA levels, suggesting that the amount of functional enzyme was determined by modulation of transcript abundance.

Original languageEnglish (US)
Pages (from-to)284-291
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume204
Issue number1
DOIs
StatePublished - Oct 15 1994
Externally publishedYes

Fingerprint

Dolichol
Glycosylation
Acetylglucosamine
Transferases
Cricetinae
Tissue
Submandibular Gland
Enzymes
Growth
Eukaryota
Gene expression
Proteins
Genes
Modulation
Gene Expression
Messenger RNA
UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Developmental regulation and tissue-specific expression of hamster dolichol-P-dependent N-acetylglucosamine-1-P transferase (GPT). / Mota, Olivia M.; Huang, George; Kukuruzinska, Maria A.

In: Biochemical and Biophysical Research Communications, Vol. 204, No. 1, 15.10.1994, p. 284-291.

Research output: Contribution to journalArticle

@article{77cb1becda8349bb82adefa0f67573b0,
title = "Developmental regulation and tissue-specific expression of hamster dolichol-P-dependent N-acetylglucosamine-1-P transferase (GPT)",
abstract = "The first enzyme in the dolichol pathway of protein N-glycosylation, dolichol-P-dependent N-acetylglucosamine-1-phosphate transferase, GPT, has been implicated in the development of a wide variety of eukaryotes. GPT is encoded by ALG7 an early growth-response gene, whose expression has been shown to affect the extent of N-glycosylation and secretion of proteins. To initiate the molecular characterization of ALG7 involvement in mammalian growth and differentiation, we have used the postnatally developing hamster submandibular gland (SMG) as an experimental paradigm. In this study we report that the ALG7 gene was differentially expressed during postnatal development and in terminally differentiated adult tissues. Throughout development, GPT activity paralleled ALG7 mRNA levels, suggesting that the amount of functional enzyme was determined by modulation of transcript abundance.",
author = "Mota, {Olivia M.} and George Huang and Kukuruzinska, {Maria A.}",
year = "1994",
month = "10",
day = "15",
doi = "10.1006/bbrc.1994.2457",
language = "English (US)",
volume = "204",
pages = "284--291",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Developmental regulation and tissue-specific expression of hamster dolichol-P-dependent N-acetylglucosamine-1-P transferase (GPT)

AU - Mota, Olivia M.

AU - Huang, George

AU - Kukuruzinska, Maria A.

PY - 1994/10/15

Y1 - 1994/10/15

N2 - The first enzyme in the dolichol pathway of protein N-glycosylation, dolichol-P-dependent N-acetylglucosamine-1-phosphate transferase, GPT, has been implicated in the development of a wide variety of eukaryotes. GPT is encoded by ALG7 an early growth-response gene, whose expression has been shown to affect the extent of N-glycosylation and secretion of proteins. To initiate the molecular characterization of ALG7 involvement in mammalian growth and differentiation, we have used the postnatally developing hamster submandibular gland (SMG) as an experimental paradigm. In this study we report that the ALG7 gene was differentially expressed during postnatal development and in terminally differentiated adult tissues. Throughout development, GPT activity paralleled ALG7 mRNA levels, suggesting that the amount of functional enzyme was determined by modulation of transcript abundance.

AB - The first enzyme in the dolichol pathway of protein N-glycosylation, dolichol-P-dependent N-acetylglucosamine-1-phosphate transferase, GPT, has been implicated in the development of a wide variety of eukaryotes. GPT is encoded by ALG7 an early growth-response gene, whose expression has been shown to affect the extent of N-glycosylation and secretion of proteins. To initiate the molecular characterization of ALG7 involvement in mammalian growth and differentiation, we have used the postnatally developing hamster submandibular gland (SMG) as an experimental paradigm. In this study we report that the ALG7 gene was differentially expressed during postnatal development and in terminally differentiated adult tissues. Throughout development, GPT activity paralleled ALG7 mRNA levels, suggesting that the amount of functional enzyme was determined by modulation of transcript abundance.

UR - http://www.scopus.com/inward/record.url?scp=0028113251&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028113251&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1994.2457

DO - 10.1006/bbrc.1994.2457

M3 - Article

C2 - 7945372

AN - SCOPUS:0028113251

VL - 204

SP - 284

EP - 291

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -