Diagnosis of Militarily Relevant Diseases Using Oral Fluid and Saliva Antibodies

Fluorescence Polarization Immunoassay

Malford E. Cullum, Linda A. Lininger, Sylvia Z. Schade, Stanton E. Cope, James Ragain, Lloyd G. Simonson

Research output: Contribution to journalReview article

9 Citations (Scopus)

Abstract

This laboratory is developing fluorescence polarization (FP) methods as diagnostic tools to assay antibodies in saliva and other oral fluids. FP provides quantitation of molecular interaction, such as antigen-antibody binding, of a single, small-volume sample in real time and without prior separation of components such as blood cells. There is potential for wide-spread use of these homogeneous assays as noninvasive tests, especially as more compact, simplified fluorescence polarimeters become available. FP tests can be designed that are applicable to a wide spectrum of microorganisms and may be used in a clinic or far-forward deployed setting to aid in diagnosis of disease or verification of vaccination. Rapid salivary diagnostics, including FP, have been identified by the Office of Naval Research as requirements for future naval capabilities in basic and applied medical research for warfighter protection in casualty prevention. The applications of FP salivary diagnostics for determination of tuberculosis exposure and of anthrax immunization status are discussed as examples.

Original languageEnglish (US)
Pages (from-to)915-921
Number of pages7
JournalMilitary medicine
Volume168
Issue number11
StatePublished - Nov 1 2003

Fingerprint

Fluorescence Polarization Immunoassay
Fluorescence Polarization
Saliva
Antibodies
Anthrax
Biomedical Research
Immunization
Blood Cells
Vaccination
Tuberculosis
Fluorescence
Antigens
Research

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health

Cite this

Cullum, M. E., Lininger, L. A., Schade, S. Z., Cope, S. E., Ragain, J., & Simonson, L. G. (2003). Diagnosis of Militarily Relevant Diseases Using Oral Fluid and Saliva Antibodies: Fluorescence Polarization Immunoassay. Military medicine, 168(11), 915-921.

Diagnosis of Militarily Relevant Diseases Using Oral Fluid and Saliva Antibodies : Fluorescence Polarization Immunoassay. / Cullum, Malford E.; Lininger, Linda A.; Schade, Sylvia Z.; Cope, Stanton E.; Ragain, James; Simonson, Lloyd G.

In: Military medicine, Vol. 168, No. 11, 01.11.2003, p. 915-921.

Research output: Contribution to journalReview article

Cullum, ME, Lininger, LA, Schade, SZ, Cope, SE, Ragain, J & Simonson, LG 2003, 'Diagnosis of Militarily Relevant Diseases Using Oral Fluid and Saliva Antibodies: Fluorescence Polarization Immunoassay', Military medicine, vol. 168, no. 11, pp. 915-921.
Cullum, Malford E. ; Lininger, Linda A. ; Schade, Sylvia Z. ; Cope, Stanton E. ; Ragain, James ; Simonson, Lloyd G. / Diagnosis of Militarily Relevant Diseases Using Oral Fluid and Saliva Antibodies : Fluorescence Polarization Immunoassay. In: Military medicine. 2003 ; Vol. 168, No. 11. pp. 915-921.
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