Dietary antioxidant micronutrients alter mucosal inflammatory risk in a murine model of genetic and microbial susceptibility

Joseph Pierre, Reinhard Hinterleitner, Romain Bouziat, Nathan A. Hubert, Vanessa Leone, Jun Miyoshi, Bana Jabri, Eugene B. Chang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Inflammatory bowel diseases (IBD) are caused by the convergence of microbial, environmental, and genetic factors. Diet significantly alters these interactions by affecting both the host and microbiome. Using a mucosal inflammatory model that resembles the human condition of ileal pouchitis, we investigated the effects of Control (CONT) or Antioxidant (AOX) diet, containing pharmacologically relevant levels of 4 micronutrients, on disease risk in wild-type and IL-10 −/− animals following surgical self-filling (SF) ileal blind loop placement. Although no differences were found in body weight change or survival, IL-10 −/− CONT animals had significantly larger lymphoid organs compared with IL-10 −/− AOX or with WT. SF loops from IL-10 −/− CONT loop mucosa demonstrated histological inflammation, characterized by goblet cell depletion, increased mucosal myeloperoxidase (MPO), and elevated IFNγ TNFα and IL-17α gene expression, which AOX attenuated. AOX elevated luminal IgA in IL-10 −/− animals, but not significantly in WT. In IL-10 −/− animals, AOX significantly decreased the percentage of CD4 + T-bet and CD4 + RORγ T-cells compared with CONT, with no changes in CD4 + Foxp3+ Treg cells. 16S rRNA gene sequencing demonstrated AOX increased microbial alpha diversity compared with CONT in both genotypes. Notably, colonizing germ-free IL-10 −/− hosts with CONT bacterial communities, but not AOX, recapitulated the inflammatory phenotype. Collectively, these findings highlight that common dietary antioxidant micronutrients reshape the gut microbial community to mitigate intestinal inflammatory profiles in genetically susceptible hosts. Insights into the dietary-immune-microbial nexus may improve understanding for recurrent inflammatory episodes in susceptible patient populations and opportunities for practical therapeutics to restore immune and microbial homeostasis.

Original languageEnglish (US)
Pages (from-to)95-104
Number of pages10
JournalJournal of Nutritional Biochemistry
Volume54
DOIs
StatePublished - Apr 1 2018

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Micronutrients
Genetic Predisposition to Disease
Interleukin-10
Antioxidants
Animals
Nutrition
Microbial Genetics
Pouchitis
Diet
Body Weight Changes
Goblet Cells
T-cells
Interleukin-17
Microbiota
Regulatory T-Lymphocytes
Inflammatory Bowel Diseases
rRNA Genes
Gene expression
Immunoglobulin A
Peroxidase

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Dietary antioxidant micronutrients alter mucosal inflammatory risk in a murine model of genetic and microbial susceptibility. / Pierre, Joseph; Hinterleitner, Reinhard; Bouziat, Romain; Hubert, Nathan A.; Leone, Vanessa; Miyoshi, Jun; Jabri, Bana; Chang, Eugene B.

In: Journal of Nutritional Biochemistry, Vol. 54, 01.04.2018, p. 95-104.

Research output: Contribution to journalArticle

Pierre, Joseph ; Hinterleitner, Reinhard ; Bouziat, Romain ; Hubert, Nathan A. ; Leone, Vanessa ; Miyoshi, Jun ; Jabri, Bana ; Chang, Eugene B. / Dietary antioxidant micronutrients alter mucosal inflammatory risk in a murine model of genetic and microbial susceptibility. In: Journal of Nutritional Biochemistry. 2018 ; Vol. 54. pp. 95-104.
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abstract = "Inflammatory bowel diseases (IBD) are caused by the convergence of microbial, environmental, and genetic factors. Diet significantly alters these interactions by affecting both the host and microbiome. Using a mucosal inflammatory model that resembles the human condition of ileal pouchitis, we investigated the effects of Control (CONT) or Antioxidant (AOX) diet, containing pharmacologically relevant levels of 4 micronutrients, on disease risk in wild-type and IL-10 −/− animals following surgical self-filling (SF) ileal blind loop placement. Although no differences were found in body weight change or survival, IL-10 −/− CONT animals had significantly larger lymphoid organs compared with IL-10 −/− AOX or with WT. SF loops from IL-10 −/− CONT loop mucosa demonstrated histological inflammation, characterized by goblet cell depletion, increased mucosal myeloperoxidase (MPO), and elevated IFNγ TNFα and IL-17α gene expression, which AOX attenuated. AOX elevated luminal IgA in IL-10 −/− animals, but not significantly in WT. In IL-10 −/− animals, AOX significantly decreased the percentage of CD4 + T-bet and CD4 + RORγ T-cells compared with CONT, with no changes in CD4 + Foxp3+ Treg cells. 16S rRNA gene sequencing demonstrated AOX increased microbial alpha diversity compared with CONT in both genotypes. Notably, colonizing germ-free IL-10 −/− hosts with CONT bacterial communities, but not AOX, recapitulated the inflammatory phenotype. Collectively, these findings highlight that common dietary antioxidant micronutrients reshape the gut microbial community to mitigate intestinal inflammatory profiles in genetically susceptible hosts. Insights into the dietary-immune-microbial nexus may improve understanding for recurrent inflammatory episodes in susceptible patient populations and opportunities for practical therapeutics to restore immune and microbial homeostasis.",
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