Dietary fatty acids fine-tune Piezo1 mechanical response

Luis O. Romero, Andrew E. Massey, Alejandro Mata Daboin, Francisco J. Sierra-Valdez, Subhash Chauhan, Julio Cordero-Morales, Valeria Vasquez

Research output: Contribution to journalArticle

Abstract

Mechanosensitive ion channels rely on membrane composition to transduce physical stimuli into electrical signals. The Piezo1 channel mediates mechanoelectrical transduction and regulates crucial physiological processes, including vascular architecture and remodeling, cell migration, and erythrocyte volume. The identity of the membrane components that modulate Piezo1 function remain largely unknown. Using lipid profiling analyses, we here identify dietary fatty acids that tune Piezo1 mechanical response. We find that margaric acid, a saturated fatty acid present in dairy products and fish, inhibits Piezo1 activation and polyunsaturated fatty acids (PUFAs), present in fish oils, modulate channel inactivation. Force measurements reveal that margaric acid increases membrane bending stiffness, whereas PUFAs decrease it. We use fatty acid supplementation to abrogate the phenotype of gain-of-function Piezo1 mutations causing human dehydrated hereditary stomatocytosis. Beyond Piezo1, our findings demonstrate that cell-intrinsic lipid profile and changes in the fatty acid metabolism can dictate the cell’s response to mechanical cues.

Original languageEnglish (US)
Article number1200
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

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fatty acids
Fatty Acids
Membranes
Unsaturated Fatty Acids
fishes
Dairy products
Erythrocyte Volume
Physiological Phenomena
membranes
Lipids
Dairy Products
lipids
Fish Oils
Force measurement
cells
Ion Channels
Cell Size
Metabolism
Fish
Cell Movement

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Dietary fatty acids fine-tune Piezo1 mechanical response. / Romero, Luis O.; Massey, Andrew E.; Mata Daboin, Alejandro; Sierra-Valdez, Francisco J.; Chauhan, Subhash; Cordero-Morales, Julio; Vasquez, Valeria.

In: Nature communications, Vol. 10, No. 1, 1200, 01.12.2019.

Research output: Contribution to journalArticle

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