Different accumulation profiles of multiple components between pericarp and seed of alpinia oxyphylla capsular fruit as determined by uflc-ms/ms

Feng Chen, Hai Long Li, Yin Feng Tan, Wei Wei Guan, Jun Qing Zhang, Yong Hui Li, Yuan Sheng Zhao, Zhen Miao Qin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Plant secondary metabolites are known to not only play a key role in the adaptation of plants to their environment, but also represent an important source of active pharmaceuticals. Alpinia oxyphylla capsular fruits, made up of seeds and pericarps, are commonly used in traditional East Asian medicines. In clinical utilization of these capsular fruits, inconsistent processing approaches (i.e., hulling pericarps or not) are employed, with the potential of leading to differential pharmacological effects. Therefore, an important question arises whether the content levels of pharmacologically active chemicals between the seeds and pericarps of A. oxyphylla are comparable. Nine secondary metabolites present in A. oxyphylla capsular fruits, including flavonoids (e.g., tectochrysin, izalpinin, chrysin, apigenin-4',7-dimethylether and kaempferide), diarylheptanoids (e.g., yakuchinone A and B and oxyphyllacinol) and sesquiterpenes (e.g., nootkatone), were regarded as representative constituents with putative pharmacological activities. This work aimed to investigate the abundance of the nine constituents in the seeds and pericarps of A. oxyphylla. Thirteen batches of A. oxyphylla capsular fruits were gathered from different production regions.Accordingly, an ultra-fast high performance liquid chromatography/quadrupole tandem mass spectrometry (UFLC-MS/MS) method was developed and validated. We found that: (1) the nine secondary metabolites were differentially concentrated in seeds and fruit capsules; (2) nootkatone is predominantly distributed in the seeds; in contrast, the flavonoids and diarylheptanoids are mainly deposited in the capsules; and (3) the content levels of the nine secondary metabolites occurring in the capsules varied greatly among different production regions, although the nootkatone levels in the seeds were comparable among production regions. These results are helpful to evaluating and elucidating pharmacological activities of A. oxyphylla capsular fruits. Additionally, it may be of interest to elucidate the mechanisms involved in the distinct accumulation profiles of these secondary metabolites between seeds and pericarps.

Original languageEnglish (US)
Pages (from-to)4510-4523
Number of pages14
JournalMolecules
Volume19
Issue number4
DOIs
StatePublished - Jan 1 2014

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Alpinia
fruits
Fruits
Seed
seeds
Fruit
Seeds
metabolites
Metabolites
profiles
Diarylheptanoids
capsules
Capsules
Pharmacology
activity (biology)
Flavonoids
East Asian Traditional Medicine
Apigenin
Sesquiterpenes
liquid chromatography

All Science Journal Classification (ASJC) codes

  • Organic Chemistry

Cite this

Different accumulation profiles of multiple components between pericarp and seed of alpinia oxyphylla capsular fruit as determined by uflc-ms/ms. / Chen, Feng; Li, Hai Long; Tan, Yin Feng; Guan, Wei Wei; Zhang, Jun Qing; Li, Yong Hui; Zhao, Yuan Sheng; Qin, Zhen Miao.

In: Molecules, Vol. 19, No. 4, 01.01.2014, p. 4510-4523.

Research output: Contribution to journalArticle

Chen, Feng ; Li, Hai Long ; Tan, Yin Feng ; Guan, Wei Wei ; Zhang, Jun Qing ; Li, Yong Hui ; Zhao, Yuan Sheng ; Qin, Zhen Miao. / Different accumulation profiles of multiple components between pericarp and seed of alpinia oxyphylla capsular fruit as determined by uflc-ms/ms. In: Molecules. 2014 ; Vol. 19, No. 4. pp. 4510-4523.
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