Differential distribution and functional impact of BK channel beta1 subunits across mesenteric, coronary, and different cerebral arteries of the rat

Guruprasad Kuntamallappanavar, Shivantika Bisen, Anna Bukiya, Alejandro Dopico

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Large conductance, Ca2+ i- and voltage-gated K+ (BK) channels regulate myogenic tone and, thus, arterial diameter. In smooth muscle (SM), BK channels include channel-forming α and auxiliary β1 subunits. BK β1 increases the channel’s Ca2+ sensitivity, allowing BK channels to negatively feedback on depolarization-induced Ca2+ entry, oppose SM contraction and favor vasodilation. Thus, endothelial-independent vasodilation can be evoked though targeting of SM BK β1 by endogenous ligands, including lithocholate (LCA). Here, we investigated the expression of BK β1 across arteries of the cerebral and peripheral circulations, and the contribution of such expression to channel function and BK β1-mediated vasodilation. Data demonstrate that endothelium-independent, BK β1-mediated vasodilation by LCA is larger in coronary (CA) and basilar (BA) arteries than in anterior cerebral (ACA), middle cerebral (MCA), posterior cerebral (PCA), and mesenteric (MA) arteries, all arterial segments having a similar diameter. Thus, differential dilation occurs in extracranial arteries which are subjected to similar vascular pressure (CA vs. MA) and in arteries that irrigate different brain regions (BA vs. ACA, MCA, and PCA). SM BK channels from BA and CA displayed increased basal activity and LCA responses, indicating increased BK β1 functional presence. Indeed, in the absence of detectable changes in BK α, BA and CA myocytes showed an increased location of BK β1 in the plasmalemma/subplasmalemma. Moreover, these myocytes distinctly showed increased BK β1 messenger RNA (mRNA) levels. Supporting a major role of enhanced BK β1 transcripts in artery dilation, LCA-induced dilation of MCA transfected with BK β1 complementary DNA (cDNA) was as high as LCA-induced dilation of untransfected BA or CA.

Original languageEnglish (US)
Pages (from-to)263-277
Number of pages15
JournalPflugers Archiv European Journal of Physiology
Volume469
Issue number2
DOIs
StatePublished - Feb 1 2017

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Lithocholic Acid
Large-Conductance Calcium-Activated Potassium Channels
Cerebral Arteries
Rats
Vasodilation
Smooth Muscle
Muscle
Dilatation
Arteries
Muscle Cells
Cerebrovascular Circulation
Voltage-Gated Potassium Channels
Basilar Artery
Mesenteric Arteries
Depolarization
Muscle Contraction
Endothelium
Blood Vessels
Brain
Coronary Vessels

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

Differential distribution and functional impact of BK channel beta1 subunits across mesenteric, coronary, and different cerebral arteries of the rat. / Kuntamallappanavar, Guruprasad; Bisen, Shivantika; Bukiya, Anna; Dopico, Alejandro.

In: Pflugers Archiv European Journal of Physiology, Vol. 469, No. 2, 01.02.2017, p. 263-277.

Research output: Contribution to journalArticle

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