Differential distribution of nicotinamide adenine dinucleotide phosphate-diaphorase and neural nitric oxide synthase in the rat choroid plexus. A histochemical and immunocytochemical study

G. Sancesario, Anton Reiner, G. Figueredo-Cardenas, M. Morello, G. Bernardi

Research output: Contribution to journalArticle

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Abstract

This study used NADPH diaphorase (NADPHd) histochemistry and neuronal nitric oxide synthase immunocytochemistry to examine the localization of nitric oxide synthase in the choroid plexus of the lateral ventricles and the fourth ventricle of rat brain. That the NADPHd reaction product in choroid plexus was specific to nitric oxide synthase was evaluated: (i) by comparison to immunocytochemical labelling for nitric oxide synthase; and (ii) by comparing NADPHd histochemical staining in choroid plexus and brain (rich in nitric oxide synthase-positive and NADPHd-positive neurons) in the presence or absence of iodonium diphenyl or dichlorophenolindophenol, two potent albeit non-selective inhibitors of nitric oxide synthase activity. In brain, NADPHd histochemistry homogeneously stained neuronal cell bodies, axons and dendrites, while it produced particulate cytoplasmic staining of all epithelial cells in the choroid plexuses of the lateral and fourth ventricles. Within the choroid plexus of the lateral ventricles, NADPHd-positive nerve fibres were also observed around blood vessels and coursing among the epithelial cells. The distribution of immunoreactivity for nitric oxide synthase in brain and in nerve fibres in the choroid plexuses of the lateral ventricles resembled the distribution of histochemical labelling for NADPHd. Choroid plexus epithelial cells were, however, devoid of nitric oxide synthase immunoreactivity. Consistent with this, iodonium diphenyl and dichlorophenolindophenol (0.1 mM) inhibited NADPHd histochemical staining in brain neurons and in choroid plexus nerve fibres, but not in choroid plexus epithelial cells. These results demonstrate that the choroid plexus of the lateral ventricles in rat brain is innervated by nitric oxide synthase-positive nerve fibres. These nitric oxide synthase-positive nerve fibres may have an important role in the regulation of cerebrospinal fluid balance. Although choroid plexus epithelial cells contain an enzyme with NADPHd activity, this enzyme is not nitric oxide synthase.

Original languageEnglish (US)
Pages (from-to)365-375
Number of pages11
JournalNeuroscience
Volume72
Issue number2
DOIs
StatePublished - Jan 1 1996

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Choroid Plexus
NADPH Dehydrogenase
NADP
Nitric Oxide Synthase
Lateral Ventricles
Nerve Fibers
Epithelial Cells
Brain
2,6-Dichloroindophenol
Fourth Ventricle
Staining and Labeling
Neurons
Nitric Oxide Synthase Type I
Water-Electrolyte Balance
Enzymes
Dendrites
Blood Vessels
Axons
Cerebrospinal Fluid
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Differential distribution of nicotinamide adenine dinucleotide phosphate-diaphorase and neural nitric oxide synthase in the rat choroid plexus. A histochemical and immunocytochemical study. / Sancesario, G.; Reiner, Anton; Figueredo-Cardenas, G.; Morello, M.; Bernardi, G.

In: Neuroscience, Vol. 72, No. 2, 01.01.1996, p. 365-375.

Research output: Contribution to journalArticle

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